Enzymatic production of human milk fat substitutes containing γ-linolenic acid: Optimization of reactions by response surface methodology

Nese Sahin, Casimir C. Akoh*, Artemis Karaali

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Structured lipids resembling human milk fat and containing GLA were synthesized by an enzymatic interesterification between tripalmitin, hazelnut oil FA, and GLA in n-hexane. Commercially immobilized 1,3-specific lipases, Lipozyme® RM IM and Lipozyme® TL IM, were used as the biocatalysts. The effect of these enzymes on the incorporation levels was investigated. A central composite design with five levels and three factors - substrate ratio, reaction temperature, and time - were used to model and optimize the reaction conditions via response surface methodology. Good quadratic models were obtained for the incorporation of GLA (response 1) and oleic acid (response 2) by multiple regression and backward elimination. The determination coefficient (R 2) values for the models were found to be 0.92 and 0.94 for the reactions catalyzed by Lipozyme RM IM, and 0.92 and 0.88 for the reactions catalyzed by Lipozyme TL IM, respectively. The optimal conditions generated from the models for the targeted GLA (10%) and oleic acid (45%) incorporation were 14.8 mol/mol, 55°C, and 24 h; 14 mol/mol, 55°C, and 24 h for substrate ratio (moles total FA/mol tripalmitin), temperature and time for the reactions catalyzed by Lipozyme RM IM and Lipozyme TL IM, respectively. Human milk fat substitutes containing GLA that can be included in infant formulas were successfully produced using both Lipozyme RM IM and Lipozyme TL IM enzymes. The effect of the two enzymes on the incorporation of GLA and oleic acid were found to be similar.

Original languageEnglish
Pages (from-to)549-557
Number of pages9
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume82
Issue number8
DOIs
Publication statusPublished - 2005

Funding

Nese Sahin was supported by a scholarship grant under the scope of the NATO Science Fellowships Programme by the Scientific and Technical Research Council of Turkey (TUBITAK) and by the University of Georgia Food Science Research fund.

FundersFunder number
TUBITAK
North Atlantic Treaty Organization
University of Georgia
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu

    Keywords

    • Enzymatic interesterification
    • GLA
    • Hazelnut oil
    • Human milk fat substitutes
    • Response surface methodology
    • Sn-1,3-specific lipase

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