Enrichment of lignocellulose-degrading microbial communities from natural and engineered methanogenic environments

Emine Gozde Ozbayram*, Sabine Kleinsteuber, Marcell Nikolausz, Bahar Ince, Orhan Ince

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The aim of this study was to develop an effective bioaugmentation concept for anaerobic digesters treating lignocellulosic biomass such as straw. For that purpose, lignocellulose-degrading methanogenic communities were enriched on wheat straw from cow and goat rumen fluid as well as from a biogas reactor acclimated to lignocellulosic biomass (sorghum as mono-substrate). The bacterial communities of the enriched cultures and the different inocula were examined by 454 amplicon sequencing of 16S rRNA genes while the methanogenic archaeal communities were analyzed by terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of the mcrA gene. Bacteroidetes was the most abundant phylum in all samples. Within the Bacteroidetes phylum, Bacteroidaceae was the most abundant family in the rumen-derived enrichment cultures, whereas Porphyromonadaceae was the predominant one in the reactor-derived culture. Additionally, the enrichment procedure increased the relative abundance of Ruminococcaceae (phylum: Firmicutes) in all cultures. T-RFLP profiles of the mcrA gene amplicons highlighted that the ruminal methanogenic communities were composed of hydrogenotrophic methanogens dominated by the order Methanobacteriales regardless of the host species. The methanogenic communities changed significantly during the enrichment procedure, but still the strict hydrogenotrophic Methanobacteriales and Methanomicrobiales were the predominant orders in the enrichment cultures. The bioaugmentation potential of the enriched methanogenic cultures will be evaluated in further studies.

Original languageEnglish
Pages (from-to)1035-1043
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume102
Issue number2
DOIs
Publication statusPublished - 1 Jan 2018

Bibliographical note

Publisher Copyright:
© 2017, Springer-Verlag GmbH Germany, part of Springer Nature.

Funding

The authors would like to acknowledge Prof. Alexander Starke and Dr. Matthias Kaiser from the Veterinary Faculty of Leipzig University for providing access to the ruminants and support in rumen sampling. The authors also thank Birke Brumme (UFZ) for technical assistance in anaerobic cultivation, Ute Lohse (UFZ) for assistance in pyrosequencing and Athaydes Francisco Leite (UFZ) and Canan Karakoç (UFZ) for their help in sequence data analysis. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The authors declare that there are no conflicts of interest. Funding Emine Gozde Ozbayram was supported by the Research Fellowship Programme of the Scientific and Technological Research Council of Turkey (grant no. 2214A).

FundersFunder number
Veterinary Faculty of Leipzig University
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu2214A
Helmholtz-Zentrum für Umweltforschung

    Keywords

    • Amplicon sequencing
    • Cow rumen
    • Goat rumen
    • mcrA
    • Sorghum
    • T-RFLP

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