Enhancing magnetic resonance imaging tumor detection with fluorescence intensity and lifetime imaging

Ahmet Erten; David Hall; Carl Hoh; Hop S. Tran Cao; Sharmeela Kaushal; Sadik Esener; Robert M. Hoffman; Michael Bouvet; James Chen; Santosh Kesari; Milan Makale

doi:10.1117/1.3509111

Enhancing magnetic resonance imaging tumor detection with fluorescence intensity and lifetime imaging

Ahmet Erten, David Hall, Carl Hoh, Hop S. Tran Cao, Sharmeela Kaushal, Sadik Esener, Robert M. Hoffman, Michael Bouvet, James Chen, Santosh Kesari, Milan Makale^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Early detection is important for many solid cancers but the images provided by ultrasound, magnetic resonance imaging (MRI), and computed tomography applied alone or together, are often not sufficient for decisive early screeningdiagnosis. We demonstrate that MRI augmented with fluorescence intensity (FI) substantially improves detection. Early stage murine pancreatic tumors that could not be identified by blinded, skilled observers using MRI alone, were easily identified with MRI along with FI images acquired with photomultiplier tube detection and offset laser scanning. Moreover, we show that fluorescence lifetime (FLT) imaging enables positive identification of the labeling fluorophore and discriminates it from surrounding tissue autofluorescence. Our data suggest combined-modality imaging with MRI, FI, and FLT can be used to screen and diagnose early tumors.

Original language	English
Article number	066012
Journal	Journal of Biomedical Optics
Volume	15
Issue number	6
DOIs	https://doi.org/10.1117/1.3509111
Publication status	Published - Nov 2010
Externally published	Yes

Funding

The authors wish to acknowledge the excellent editorial assistance of Ms. Charlene M. Cooper, and the expert imaging assistance provided by Mr. Salman Farshchi-Haydari. This work was in part funded by the National Cancer Institute Grant No. CA109949 and the American Cancer Society Grant No. RSG-05-037-01-CCE (to M.B.), T32 training Grant No. CA121938 (to H.S.T.C.), National Cancer Institute Grant No. CA132971 (to M.B. and AntiCancer Inc.), National Institutes of Health (NIH) Grant No. S10 RR22599-01A1 (to D.J.H) and NIH Grant No. P50 CA128346-01A1.

Funders	Funder number
AntiCancer Inc.
National Institutes of Health	S10 RR22599-01A1
American Cancer Society	CA121938, RSG-05-037-01-CCE
National Cancer Institute	CA109949, R01CA132971

Keywords

cancer detection
fluorescence intensity imaging
magnetic resonance imaging
orthopedic models
pancreatic cancer
red fluorescent protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/1.3509111

Cite this

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abstract = "Early detection is important for many solid cancers but the images provided by ultrasound, magnetic resonance imaging (MRI), and computed tomography applied alone or together, are often not sufficient for decisive early screeningdiagnosis. We demonstrate that MRI augmented with fluorescence intensity (FI) substantially improves detection. Early stage murine pancreatic tumors that could not be identified by blinded, skilled observers using MRI alone, were easily identified with MRI along with FI images acquired with photomultiplier tube detection and offset laser scanning. Moreover, we show that fluorescence lifetime (FLT) imaging enables positive identification of the labeling fluorophore and discriminates it from surrounding tissue autofluorescence. Our data suggest combined-modality imaging with MRI, FI, and FLT can be used to screen and diagnose early tumors.",

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AU - Erten, Ahmet

AU - Hall, David

AU - Hoh, Carl

AU - Tran Cao, Hop S.

AU - Kaushal, Sharmeela

AU - Esener, Sadik

AU - Hoffman, Robert M.

AU - Bouvet, Michael

AU - Chen, James

AU - Kesari, Santosh

AU - Makale, Milan

PY - 2010/11

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N2 - Early detection is important for many solid cancers but the images provided by ultrasound, magnetic resonance imaging (MRI), and computed tomography applied alone or together, are often not sufficient for decisive early screeningdiagnosis. We demonstrate that MRI augmented with fluorescence intensity (FI) substantially improves detection. Early stage murine pancreatic tumors that could not be identified by blinded, skilled observers using MRI alone, were easily identified with MRI along with FI images acquired with photomultiplier tube detection and offset laser scanning. Moreover, we show that fluorescence lifetime (FLT) imaging enables positive identification of the labeling fluorophore and discriminates it from surrounding tissue autofluorescence. Our data suggest combined-modality imaging with MRI, FI, and FLT can be used to screen and diagnose early tumors.

AB - Early detection is important for many solid cancers but the images provided by ultrasound, magnetic resonance imaging (MRI), and computed tomography applied alone or together, are often not sufficient for decisive early screeningdiagnosis. We demonstrate that MRI augmented with fluorescence intensity (FI) substantially improves detection. Early stage murine pancreatic tumors that could not be identified by blinded, skilled observers using MRI alone, were easily identified with MRI along with FI images acquired with photomultiplier tube detection and offset laser scanning. Moreover, we show that fluorescence lifetime (FLT) imaging enables positive identification of the labeling fluorophore and discriminates it from surrounding tissue autofluorescence. Our data suggest combined-modality imaging with MRI, FI, and FLT can be used to screen and diagnose early tumors.

KW - cancer detection

KW - fluorescence intensity imaging

KW - magnetic resonance imaging

KW - orthopedic models

KW - pancreatic cancer

KW - red fluorescent protein

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Enhancing magnetic resonance imaging tumor detection with fluorescence intensity and lifetime imaging

Abstract

Funding

Keywords

UN SDGs

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