Enhanced functions of osteoblasts on nanophase ceramics

Thomas J. Webster, Celaletdin Ergun, Robert H. Doremus, Richard W. Siegel, Rena Bizios*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1321 Citations (Scopus)

Abstract

Select functions of osteoblasts (bone-forming cells) on nanophase (materials with grain sizes less than 100nm) alumina, titania, and hydroxyapatite (HA) were investigated using in vitro cellular models. Compared to conventional ceramics, surface occupancy of osteoblast colonies was significantly less on all nanophase ceramics tested in the present study after 4 and 6 days of culture. Osteoblast proliferation was significantly greater on nanophase alumina, titania, and HA than on conventional formulations of the same ceramic after 3 and 5 days. More importantly, compared to conventional ceramics, synthesis of alkaline phosphatase and deposition of calcium-containing mineral was significantly greater by osteoblasts cultured on nanophase than on conventional ceramics after 21 and 28 days. The results of the present study provided the first evidence of enhanced long-term (on the order of days to weeks) functions of osteoblasts cultured on nanophase ceramics; in this manner, nanophase ceramics clearly represent a unique and promising class of orthopaedic/dental implant formulations with improved osseointegrative properties. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)1803-1810
Number of pages8
JournalBiomaterials
Volume21
Issue number17
DOIs
Publication statusPublished - Sept 2000
Externally publishedYes

Keywords

  • Alkaline phosphatase
  • Cell proliferation
  • Mineralization
  • Nanoceramics
  • Orthopaedic/dental
  • Osteoblasts

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