Embryo viability indexing using Raman spectroscopy of spent culture media

Uğur Parlatan; Günay Başar; Nima Bavili; Harika Yumru; Fatih Sag; Sibel Bulgurcuoglu; Ercan Bastu

doi:10.1080/00387010.2016.1198814

Embryo viability indexing using Raman spectroscopy of spent culture media

Uğur Parlatan^*, Günay Başar, Nima Bavili, Harika Yumru, Fatih Sag, Sibel Bulgurcuoglu, Ercan Bastu

^*Corresponding author for this work

Department of Physics Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm^-1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.

Original language	English
Pages (from-to)	458-463
Number of pages	6
Journal	Spectroscopy Letters
Volume	49
Issue number	7
DOIs	https://doi.org/10.1080/00387010.2016.1198814
Publication status	Published - 17 Jun 2016

Bibliographical note

Publisher Copyright:
© 2016 Taylor & Francis.

Keywords

Band component analysis
Embryo viability
In vitro fertilization
Raman spectroscopy

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10.1080/00387010.2016.1198814

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abstract = "Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm-1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.",

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AU - Parlatan, Uğur

AU - Başar, Günay

AU - Bavili, Nima

AU - Yumru, Harika

AU - Sag, Fatih

AU - Bulgurcuoglu, Sibel

AU - Bastu, Ercan

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AB - Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm-1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.

KW - Band component analysis

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KW - In vitro fertilization

KW - Raman spectroscopy

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JO - Spectroscopy Letters

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Embryo viability indexing using Raman spectroscopy of spent culture media

Abstract

Bibliographical note

Keywords

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