Abstract
The effect of the electric field strength on the Escherichia coli (E. coli) bacteria deposition into the pores of porous silicon was investigated by impedance spectroscopy technique. The main idea behind this approach is that negatively charged E. coli bacteria can be deposited into the pores available on the surface of porous silicon upon the application of a high electric field. For this purpose, the influence of the E. coli concentration on the impedance spectra of the anodically formed porous silicon under various electric fields between 0 and 10 kV/cm was investigated. In addition, the effect of the application time of a constant electric field of 12 kV/cm on the impedance spectra of porous silicon exposed to living and dead bacterial cells was also investigated. The results reported in this study indicate that the number of live E. coli bacteria deposited into the pores of porous silicon can be controlled by the applied electric field strength. On the other hand, it was found that there is no considerable effect of the dead E. coli cell concentration on the recorded impedance spectra of the porous silicon based sensor platform.
| Original language | English |
|---|---|
| Pages (from-to) | 96-101 |
| Number of pages | 6 |
| Journal | Journal of Microbiological Methods |
| Volume | 161 |
| DOIs | |
| Publication status | Published - Jun 2019 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2019 Elsevier B.V.
Funding
This work was supported by the Research Fund of Yildiz Technical University (Project no. 2015-01-01-KAP03 ). The authors also thank Dr. F. Çalışlar (Istanbul University, Cerrahpaşa Medicine Faculty, Microbiology Laboratory) for supplying bacterial cells.
| Funders | Funder number |
|---|---|
| Yildiz Teknik Üniversitesi | 2015-01-01-KAP03 |
Keywords
- E. coli
- Electric field
- Impedance spectroscopy
- Porous silicon