Abstract
In this study, methanogenic Archaea diversity in an aerated landfill bioreactor filled with co-disposed incineration bottom ashes and shredded incombustible wastes was monitored and analyzed as a function of time using molecular techniques. Besides, the effects of insufficient air injection on the bioreactor performance and methanogenic diversity were evaluated thoroughly. Results indicated that rapid bio-stabilization of solid waste are possible with aerated landfill bioreactor at various oxygen and oxidation reduction potential levels. Slot-blot hybridization results of leachate samples collected from aerated landfill bioreactor showed that archaeal and bacterial activities increased as stabilization accelerated and bacterial populations constituted almost 95% of all microorganisms. The results of slot-blot hybridization and phylogenetic analysis based on 16S rRNA gene revealed that Methanobacteriales and Methanomicrobiales were dominant species at the beginning while substituted by Methanosarcina-related methanogens close to the end of the operation of bioreactor.
| Original language | English |
|---|---|
| Pages (from-to) | 258-265 |
| Number of pages | 8 |
| Journal | Journal of Hazardous Materials |
| Volume | 142 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2 Apr 2007 |
| Externally published | Yes |
Funding
The authors gratefully acknowledge the contributions by staff at Yorii Environmental Management Center (Saitama, Japan) and Mr. Toshiro Oshikata and Mr. Masaaki Ebihara of Taisei Corporation (Tokyo, Japan) for the construction of the test cells. This work was financially supported by Ministry of Environment, Japan (Project No.: K1535) and TUBITAK (Project Nos.: ICTAG A045, A051 and Ç106).
| Funders | Funder number |
|---|---|
| TUBITAK | A051, ICTAG A045, Ç106 |
| Ministry of the Environment, Government of Japan | K1535 |
Keywords
- Aeration
- Incineration ash
- Landfill
- Methanogen
- Molecular methods
