TY - JOUR
T1 - Effects of cytokinins, gibberellic acid 3, and gibberellic acid 4/7 on in vitro growth, morphological traits, and content of steviol glycosides in Stevia rebaudiana
AU - Pazuki, Arman
AU - Aflaki, Fatemeh
AU - Yücesan, Buhara
AU - Gürel, Songül
N1 - Publisher Copyright:
© 2019 Elsevier Masson SAS
PY - 2019/4
Y1 - 2019/4
N2 - Steviol glycosides (SGs) and gibberellic acids share a part of their biosynthesis pathways. Despite the widespread studies on the effect of gibberellic acid 3 (GA 3 ), the effect of gibberellic acid 4 and 7 (GA 4/7 ) on Stevia rebaudiana has never been studied. This study aimed at a comparative evaluation of different hormone effects, i.e., 1 mg L −1 GA 4/7 , 1 mg L −1 GA 3 , or 0.5 mg L −1 kinetin and 0.5 mg L −1 6-benzylaminopurine (BAP) (KB 0.5), on in vitro propagation, growth, morphological properties, and content of SGs in leaf samples of stevia. In comparison with the control group (hormone-free), the treatments of KB 0.5 or GA 3 produced the highest biomasses and largest leaf areas. The three hormonal treatments produced a similar number of leaves, the ratio of fresh to dry weight, and leaf length. GA 4/7 -treated explants produced the highest ratio of leaf area to leaf length. The effect of GA 4/7 on shoot elongation was greater than that of the control or even GA 3 . While the effect of GA 3 on rebaudioside-A (Reb-A) production was similar to that of the control (16.2 and 18.04 mg g −1 , respectively), GA 4/7 resulted in a lower amount of it (13.31 mg g −1 ). Except for GA 4/7 , which induced more stevioside accumulation, the treatments’ effects were comparable to that of the control. The ratio of stevioside to Reb-A was the highest for GA 4/7 (2.62), followed by GA 3 (1.93), and then the two others. Sum of Reb-A and stevioside content was not changed by the use of any of the treatments.
AB - Steviol glycosides (SGs) and gibberellic acids share a part of their biosynthesis pathways. Despite the widespread studies on the effect of gibberellic acid 3 (GA 3 ), the effect of gibberellic acid 4 and 7 (GA 4/7 ) on Stevia rebaudiana has never been studied. This study aimed at a comparative evaluation of different hormone effects, i.e., 1 mg L −1 GA 4/7 , 1 mg L −1 GA 3 , or 0.5 mg L −1 kinetin and 0.5 mg L −1 6-benzylaminopurine (BAP) (KB 0.5), on in vitro propagation, growth, morphological properties, and content of SGs in leaf samples of stevia. In comparison with the control group (hormone-free), the treatments of KB 0.5 or GA 3 produced the highest biomasses and largest leaf areas. The three hormonal treatments produced a similar number of leaves, the ratio of fresh to dry weight, and leaf length. GA 4/7 -treated explants produced the highest ratio of leaf area to leaf length. The effect of GA 4/7 on shoot elongation was greater than that of the control or even GA 3 . While the effect of GA 3 on rebaudioside-A (Reb-A) production was similar to that of the control (16.2 and 18.04 mg g −1 , respectively), GA 4/7 resulted in a lower amount of it (13.31 mg g −1 ). Except for GA 4/7 , which induced more stevioside accumulation, the treatments’ effects were comparable to that of the control. The ratio of stevioside to Reb-A was the highest for GA 4/7 (2.62), followed by GA 3 (1.93), and then the two others. Sum of Reb-A and stevioside content was not changed by the use of any of the treatments.
KW - Diterpene steviol glycosides
KW - Gibberellin
KW - Rebaudioside-A
KW - Stevia rebaudiana
KW - Stevioside
UR - http://www.scopus.com/inward/record.url?scp=85061626067&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2019.02.009
DO - 10.1016/j.plaphy.2019.02.009
M3 - Article
C2 - 30784987
AN - SCOPUS:85061626067
SN - 0981-9428
VL - 137
SP - 154
EP - 161
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -