Abstract
The effects of antimicrotubule agents on haploid embryo formation from unpollinated ovules of sugar beet (Beta vulgaris L.) were investigated. The antimitotic agent colchicine (at 100 and 150 mg/l) and trifluralin (at 5.0 mg/l) increased the frequency of haploid embryo formation whereas pronamide (at 76.9 and 128.2 mg/l) and trifluralin (at 3.4 mg/l) decreased. Ovules that were non-treated with antimicrotuble agents (i.e., ovules of the control treatment) produced higher percentages of haploid embryos (4.25%) when compared to the pronamide and trifluralin at 3.4 mg/l concentration. Toxic effects of these agents on embryo formation from ovules were evident. A significant genotypic variation among the lines used was observed. The line M4 produced the highest yield with a mean of 14.71% haploid embryo production while the line M2 producing no embryos at all.
| Original language | English |
|---|---|
| Pages (from-to) | 97-101 |
| Number of pages | 5 |
| Journal | Biotechnology and Biotechnological Equipment |
| Volume | 17 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 2003 |
| Externally published | Yes |