Abstract
The study addressed acetate utilization by an acclimated mixed microbial culture under different growth conditions. It explored changes in the composition of the microbial community and variable process kinetics induced by different culture history. Sequencing batch reactors were operated at steady-state at different sludge ages of two and ten days. Microbial population structure was determined using high-throughput sequencing of 16S rRNA genes. Parallel batch experiments were conducted with acclimated biomass for respirometric analyses. A lower sludge age sustained a different community, which also reflected as variable kinetics for microbial growth and biopolymer storage. The maximum growth rate was observed to change from 3.9/d to 8.5/d and the substrate storage rate from 3.5/d to 5.9/d when the sludge age was decreased from 10. d to 2.0. d. Results challenge the basic definition of heterotrophic biomass in activated sludge models, at least by means of variable kinetics under different growth conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 68-75 |
| Number of pages | 8 |
| Journal | Bioresource Technology |
| Volume | 143 |
| DOIs | |
| Publication status | Published - Sept 2013 |
Funding
This study was supported by the Turkish Academy of Sciences as part of Fellowship Program for Integrated Doctoral Studies. The authors would like to thank The Scientific Research Fund of Istanbul Technical University (Project No.: 34235 and Project No.: 34117 ) and The Scientific and Technological Research Council of Turkey for their supports throughout the study.
| Funders | Funder number |
|---|---|
| Türkiye Bilimsel ve Teknolojik Araştirma Kurumu | |
| Türkiye Bilimler Akademisi | |
| Istanbul Teknik Üniversitesi | 34117, 34235 |
Keywords
- Acetate
- Activated Sludge Modeling
- Microbial population
- Process kinetics
- Pyrosequencing
