Effect of oxytetracycline on biogas production and active microbial populations during batch anaerobic digestion of cow manure

Bahar Ince, Halil Coban, Gokhan Turker, Emine Ertekin*, Orhan Ince

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)

Abstract

The aim of this study was to investigate the effect of a common veterinary antibiotic in biogas plants. 20 mg/kg of oxytetracycline was intramuscularly injected into a cow and its concentration in manure, which was sampled daily during the following 20 days, was measured. A total of 20 % of the injected oxytetracycline was detected in manure. Collected manure samples on days 1, 2, 3, 5, 10, 15, and 20 were digested in triplicate serum bottles at 37 C for 30 days. Control serum bottles produced 255 ± 13 mL biogas, whereas 50-60 % inhibitions were obtained for the serum bottles operated with samples collected for the 5 days after medication. Multivariate statistics used for the evaluation of FISH results showed that Methanomicrobiales were the main methanogenic group responsible for most of the biogas production. Numbers of active Bacteria and Methanomicrobiales were negatively correlated with the presence of oxytetracycline, whereas Methanosarcinales and Methanobacteriales were less affected.

Original languageEnglish
Pages (from-to)541-546
Number of pages6
JournalBioprocess and Biosystems Engineering
Volume36
Issue number5
DOIs
Publication statusPublished - May 2013

Funding

Acknowledgments This study was financially supported by the Scientific and Technical Research Council of Turkey (TUBITAK, Project No: 109Y275). The authors are thankful to the Pharmacology and Toxicology Department of Istanbul University, Faculty of Veterinary Medicine for animal medication and manure sampling.

FundersFunder number
TUBITAK109Y275
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu

    Keywords

    • Anaerobic digestion
    • Biogas
    • Fluorescent in situ hybridization
    • Oxytetracycline
    • Redundancy analysis (RDA)

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