Effect of a single application of CPP-ACPF varnish on the prevention of erosive tooth wear: An AAS, AFM and SMH study

Berna Gokkaya*, Nil Ozbek, Zeliha Guler, Suleyman Akman, A. Sezai Sarac, Betul Kargul

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Purpose: The aim of this study was to investigate the in vitro effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride-containing varnish on prevention enamel erosive tooth wear. Materials and Methods: A total of 28 enamel samples were prepared from human molars, divided into four groups: CPP-ACPF varnish, TCP-F varnish, NaF varnish, and deionised water. For the remineralisation process stimulated human pooled saliva was used. After treatment, all enamel samples were exposed to 10 ml of Coca Cola. Ca++ release was determined by atomic absorption spectroscopy (AAS). The surface topography was evaluated by atomic force microscopy (AFM). Surface microhardness of enamel was analysed and SMHR % (surface microhardness reduction) was calculated. Data were analysed with repeated measures analysis of variance (ANOVA). Results: Deionised water demonstrated a statistically significantly higher Ca+2 release compared to those of groups NaF > fTCP > CPP-ACPF, respectively (p <0.01). All groups measured for root-mean-square-roughness (Rrms) showed a statistically significantly difference of 6 x 6 μm2 and 12 x 12 μm2 enamel area (p <0.05) compared with a negative control group. CPP-ACPF varnish showed rougher surfaces than all remineralisation groups. SMHR % of enamels were as follows: CPP-ACPF < fTCP < NaF < deionised water (p <0.01). Conclusion: According to the findings of this study; CPP-ACP containing agents have a statistically statistically significant effect on preventing dental erosion. Among these, CPP-ACPF-containing remineralisation agents have the most effect on the remineralisation process.

Original languageEnglish
Pages (from-to)311-318
Number of pages8
JournalOral Health and Preventive Dentistry
Issue number2
Publication statusPublished - 1 Apr 2020

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  • AAS
  • AFM
  • Erosion
  • SMH


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