Dual enzymes-mimic activity of nanolayered manganese-calcium oxide for fluorometric determination of metformin

There are different analytical methods available for the determination of metformin, as an oral hypoglycemic and antidiabetic drug, in biological samples. However, most of these methods suffer from some drawbacks, including high-priced materials and equipment, damaging chemical reagents, time-consuming nature, and tedious operation procedures. So, in this work a new, sensitive and simple method was reported for the detection of metformine. In this regard, nanolayered manganese-calcium oxide (NL-MnCaO₂) were synthesized and characterized using scanning electron microscopy (SEM), fourier transform infrared (FTIR) spectroscopy, and X-ray powder diffraction (XRD) techniques. Also, we studied the enzyme-like activity of synthesized particles and reported a bifunctional nanozyme, which performs the dual roles for peroxidase and catalase-mimicking. The results demonstrated the hindering effect of metformin on the peroxidase-mimic activity of NL-MnCaO₂ and this effect was increased by raising metformin concentration. So, a sensitive fluorometric detection system was designed for the analytical assay of metformin, based on the terephthalic acid (TA)-H₂O₂ reaction with NL-MnCaO₂. An acceptable linearity was observed between the metformin concentration and fluorescence quenching of the system in the range of 0.07–0.77 mM. Limit of detection (LOD) and limit of quantification (LOQ) were 0.17 μM and 0.96 μM, respectively. The proposed system was applied for the estimation of metformin concentration in serum samples by recoveries of 86.68–106%. So, the proposed fluorometric method provides some main advantages such as wide linear range, low detection limit, rapid detections, high sensitivity, and good practicability for the determination of metformin in biological samples.