Abstract
Abstract: Electrochemical properties of regorafenib (REG) were studied in 0.1 M Britton−Robinson buffer-methanol solutions (3 : 2, v/v) with pH between 3 and 8 at carbon paste electrode by cyclic and differential pulse voltammetry. The results exhibited irreversible anodic peak at about 0.90 V vs. Ag/AgCl, NaCl (3 M). The anodic peak was found to be diffusion–adsorption controlled. Mechanism of REG electrochemical reaction was studied by performing density functional theory computations and mass spectrometric analysis. A validated differential pulse voltammetry (DPV) technique for REG determination was performed. The calibration curve of REG on carbon paste electrode was linear in the concentration range of 0.5–13 μg/mL and limit of detection was 0.10 µg/mL. The recommended DPV method was used to detect REG in spiked plasma and urine specimens and average recoveries were 94%.
| Original language | English |
|---|---|
| Pages (from-to) | 691-700 |
| Number of pages | 10 |
| Journal | Journal of Analytical Chemistry |
| Volume | 75 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 May 2020 |
Bibliographical note
Publisher Copyright:© 2020, Pleiades Publishing, Ltd.
Funding
This work was supported by Scientific Research Project Coordination Unit of Istanbul University, Project numbers: PYP-17194 and BEK-2017-25714. ACKNOWLEDGMENTS We wish to thank Bayer for supplying regorafenib and TUBITAK ULAKBIM, High Performance and Grid Computing Center (TR-Grid e-Infrastructure) for the calculations reported in the theoretical part of this paper. We also thank to U?ur BOZKAYA for using Gaussian 09 program package.
| Funders | Funder number |
|---|---|
| High Performance and Grid Computing Center | |
| TUBITAK ULAKBIM | |
| Bayer | |
| Istanbul Üniversitesi | PYP-17194, BEK-2017-25714 |
Keywords
- density functional theory
- determination
- plasma
- regorafenib
- urine
- voltammetry
