Development of a novel synbiotic dark chocolate enriched with Bacillus indicus HU36, maltodextrin and lemon fiber: Optimization by response surface methodology

Özlem Erdem; Mine Gültekin-Özgüven; Ijlal Berktaş; Sevcan Erşan; H. Ezgi Tuna; Ayşe Karadaǧ; Beraat Özçelik; Gürbüz Güneş; Simon M. Cutting

doi:10.1016/j.lwt.2013.10.020

Development of a novel synbiotic dark chocolate enriched with Bacillus indicus HU36, maltodextrin and lemon fiber: Optimization by response surface methodology

Özlem Erdem, Mine Gültekin-Özgüven, Ijlal Berktaş, Sevcan Erşan, H. Ezgi Tuna, Ayşe Karadaǧ, Beraat Özçelik^*, Gürbüz Güneş, Simon M. Cutting

^*Corresponding author for this work

Department of Food Engineering

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

The aim of this study was to investigate the effects of probiotic Bacillus indicus HU36 and dietary fibers (maltodextrin and lemon fiber) addition on color and organoleptic quality properties of dark chocolate. The viability of B.indicus HU36 in dark chocolate was examined as well in the study. Three-level [1.5, 3.5, 5.5 (g/100g)], two factorial (maltodextrin, lemon fiber) Central Composite Design (CCD) was performed for developing synbiotic chocolate formulation. According to our results, B.indicus HU36 showed survival rate between 88 and 91% in samples. Descriptive sensory analysis (QDA^®) and color analysis were performed to examine the effects of factors and their levels on quality attributes and describe developed chocolates in detail. While bacteria and dietary fiber addition did not show any negative effects on product sensory and color properties; dietary fiber addition improved some sensorial features significantly i.e. sweetness, firmness and adherence, The validation of the model had been accomplished by applying the conditions generated by the RSM model. This study is the first report on the use of B.indicus HU36 in potentially probiotic chocolate production.

Original language	English
Pages (from-to)	187-193
Number of pages	7
Journal	LWT
Volume	56
Issue number	1
DOIs	https://doi.org/10.1016/j.lwt.2013.10.020
Publication status	Published - Apr 2014

Funding

The authors acknowledge the financial support of EU 7th Framework Programme acronymed Project “COLORSPORE” (Project number: 207948).

Funders	Funder number
Seventh Framework Programme	207948

Keywords

Bacillus indicus HU36
Probiotic
RSM
Sensory profiling
Synbiotic chocolate

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10.1016/j.lwt.2013.10.020

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title = "Development of a novel synbiotic dark chocolate enriched with Bacillus indicus HU36, maltodextrin and lemon fiber: Optimization by response surface methodology",

abstract = "The aim of this study was to investigate the effects of probiotic Bacillus indicus HU36 and dietary fibers (maltodextrin and lemon fiber) addition on color and organoleptic quality properties of dark chocolate. The viability of B.indicus HU36 in dark chocolate was examined as well in the study. Three-level [1.5, 3.5, 5.5 (g/100g)], two factorial (maltodextrin, lemon fiber) Central Composite Design (CCD) was performed for developing synbiotic chocolate formulation. According to our results, B.indicus HU36 showed survival rate between 88 and 91% in samples. Descriptive sensory analysis (QDA{\textregistered}) and color analysis were performed to examine the effects of factors and their levels on quality attributes and describe developed chocolates in detail. While bacteria and dietary fiber addition did not show any negative effects on product sensory and color properties; dietary fiber addition improved some sensorial features significantly i.e. sweetness, firmness and adherence, The validation of the model had been accomplished by applying the conditions generated by the RSM model. This study is the first report on the use of B.indicus HU36 in potentially probiotic chocolate production.",

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doi = "10.1016/j.lwt.2013.10.020",

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AU - Erdem, Özlem

AU - Gültekin-Özgüven, Mine

AU - Berktaş, Ijlal

AU - Erşan, Sevcan

AU - Tuna, H. Ezgi

AU - Karadaǧ, Ayşe

AU - Özçelik, Beraat

AU - Güneş, Gürbüz

AU - Cutting, Simon M.

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AB - The aim of this study was to investigate the effects of probiotic Bacillus indicus HU36 and dietary fibers (maltodextrin and lemon fiber) addition on color and organoleptic quality properties of dark chocolate. The viability of B.indicus HU36 in dark chocolate was examined as well in the study. Three-level [1.5, 3.5, 5.5 (g/100g)], two factorial (maltodextrin, lemon fiber) Central Composite Design (CCD) was performed for developing synbiotic chocolate formulation. According to our results, B.indicus HU36 showed survival rate between 88 and 91% in samples. Descriptive sensory analysis (QDA®) and color analysis were performed to examine the effects of factors and their levels on quality attributes and describe developed chocolates in detail. While bacteria and dietary fiber addition did not show any negative effects on product sensory and color properties; dietary fiber addition improved some sensorial features significantly i.e. sweetness, firmness and adherence, The validation of the model had been accomplished by applying the conditions generated by the RSM model. This study is the first report on the use of B.indicus HU36 in potentially probiotic chocolate production.

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Development of a novel synbiotic dark chocolate enriched with Bacillus indicus HU36, maltodextrin and lemon fiber: Optimization by response surface methodology

Abstract

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Keywords

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