Determination of the effect of proteoliposome concentration on Aquaporin Z incorporated nanofiltration membranes

Reyhan Sengur-Tasdemir, Enise Pekgenc, Gulsum Melike Urper-Bayram, Tulay Ergon-Can, Havva Esra Tutuncu, Bihter Zeytuncu, Nevin Gul-Karaguler, Esra Ates-Genceli, Ismail Koyuncu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We report on the fabrication of AqpZ immobilized flat sheet membranes. The effects of interfacial polymerization conditions as well as proteoliposome concentration were evaluated. Commercial AqpZ were used as positive control for cloned AqpZ. Specific permeate flux of membranes at higher proteoliposome concentrations increased up to 25 times higher than thin film composite membranes; however; MgSO4 rejection is lowered almost to 1.5%. FTIR and SEM confirm immobilization of proteoliposomes. Thermal analysis showed that increasing proteoliposome concentration has no positive effect on the incorporation of proteoliposomes into polyamide structures. On the contrary, at lower proteoliposome concentrations, incorporation of proteoliposomes was found better. When combined membrane performances were compared in terms of specific permeate flux; MgSO4 and humic rejection and flux recovery after humic acid filtration, the performance of cloned AqpZ incorporated membranes (having 0.1 mg/mL proteoliposome concentration and polyamide formed with 2 min piperazine reaction time) improved 1.7 times regarding TFC membranes. According to the results, increasing proteoliposome concentration did not improve nanofiltration membrane performance. On the contrary, lower proteoliposome concentrations were found to be more effective in increasing membrane performance.

Original languageEnglish
Pages (from-to)2229-2239
Number of pages11
JournalEnvironmental Technology (United Kingdom)
Volume41
Issue number17
DOIs
Publication statusPublished - 28 Jul 2020

Bibliographical note

Publisher Copyright:
© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.

Funding

The authors are grateful to TUBITAK: [grant number 113Y359] and TUBITAK 2211 scholarship program.  Authors thank Abdulhalim Kilic and Fatma Nese Kok for their kind helps through liposome preparation. For XRD measurements, authors thank Aysegul Derya Altinay. Some data related to commercial AqpZ were taken from MSc. thesis of Enise Pekgenc for comparison purposes of cloned AqpZ.

FundersFunder number
TUBITAK 2211 scholarship program
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu113Y359

    Keywords

    • Aquaporin Z
    • flat sheet membrane
    • interfacial polymerization
    • nanofiltration
    • proteoliposome concentration

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