Determination of phenolic compounds by ultra high liquid chromatography-tandem mass spectrometry: Applications in nuts

Mine Gültekin-Özgüven, Fatma Davarci, Ayça A. Pasli, Nalan Demir, Beraat Özçelik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

A new method for qualitative and quantitative analysis of fourteen common polyphenolic compounds (gallic acid, 3,4-dihydroxybenzoic or proto-catechuic acid, chlorogenic acid, caffeic acid, syringic acid, 2-hydroxycinnamic or o-coumaric acid, p-coumaric acid, ferulic acid, rutin, rosmarinic acid, catechin, epicatechin, kaempferol and trans-cinnamic acid) was developed using ultra high liquid chromatography (UHPLC) coupled with tandem mass spectrometry (MS/MS). Selected phenolic compounds were detected in multiple reaction monitoring (MRM) mode and determined simultaneously in a 13-min analysis time and a number of validation parameters (calibration range, limit of detection, limit of quantification, repeatability, precision, and recovery) were studied. To show the usefulness of the method, phenolic compounds were studied in nuts such as hazelnut (Corylus avellana L.), pistachio ( Pistaciae vera L.) and almond (Prunus amygdaleus L.).

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalLWT
Volume64
Issue number1
DOIs
Publication statusPublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

  • Almond
  • Flavonoids
  • Hazelnut
  • Phenolic acids
  • Pistachio

Fingerprint

Dive into the research topics of 'Determination of phenolic compounds by ultra high liquid chromatography-tandem mass spectrometry: Applications in nuts'. Together they form a unique fingerprint.

Cite this