Abstract
A novel support material containing cyclic carbonate group for facile enzyme immobilization was synthesized from [poly(styrene-co-divinylbenzene)-graft-poly(glycidyl methacrylate)] “[PS-co-DVB-g-P(GMA)]” microsphere under carbon dioxide atmosphere. It was named as (poly(styrene-co-divinylbenzene)-graft-poly(cyclic-carbonate methacrylate)] “[PS-co-DVB-g-P(CCMA)]” microsphere. Laccase was covalently immobilized using the microspheres carrying cyclic epoxy and cyclic carbonate groups. The amount of enzyme loading on the cyclic carbonate groups of the microspheres was 47.8 mg/g. The immobilized enzyme was used for degradation of Bisphenol A, and Congo Red dye in packed bed reactor. The immobilized laccase preserved its initial activity about 67.5% and 93.0% for degradation of Bisphenol A and Conge Red, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 407-417 |
| Number of pages | 11 |
| Journal | Journal of Industrial and Engineering Chemistry |
| Volume | 60 |
| DOIs | |
| Publication status | Published - 25 Apr 2018 |
Bibliographical note
Publisher Copyright:© 2017 The Korean Society of Industrial and Engineering Chemistry
Funding
This work was supported by a project grant number 113Z427 from The Scientific and Technological Research Council of Turkey . We would like to thank Dr. Bekir Salih and Dr. Omur Çelikbicak for MALDI-Tof-MS analysis, Hacettepe University, Department of Chemistry.
| Funders | Funder number |
|---|---|
| Türkiye Bilimsel ve Teknolojik Araştirma Kurumu |
Keywords
- Endocrine disturbance
- Environmental biotechnology
- Immobilized laccase
- Nano-structured materials
- Packed bed reactor
