Covalent immobilization of Aspergillus niger amyloglucosidase (ANAG) with ethylenediamine-functionalized and glutaraldehyde-activated active carbon (EFGAAC) obtained from sesame seed shell

Yakup Aslan*, Yousif Mohammed Sharif, Ömer Şahin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

This study was aimed the covalently immobilization of Aspergillus niger amyloglucosidase (ANAG) onto activated carbon (AC) obtained from sesame seed shell. AC was firstly functionalized with ethylenediamine, and after then activated with glutaraldehyde. 99.80% immobilization yield and 99.83% activity yield were obtained as the result of optimization of immobilization conditions (pH and molarity of immobilization buffer, AC amount, and reaction time). The optimum pH (5.5) and the optimum temperature range (55–60 °C) for ANAG were not affected by immobilization. After immobilization, Vmax value decreased from 1464.1 μmol D-glucose/L.min to 1342.3 μmol D-glucose/L.min, while Km value decreased from 116.3 g maltodextrin/L to 109.9 g maltodextrin/L. The immobilized enzyme retained 99.30% and 98.30% of its initial activity, respectively after twenty repeated uses and after twenty days of storage in 5 mL sodium phosphate buffer (0.1 M, pH 5.5) at +4 °C in a refrigerator. Finally, glucose syrup was produced from maltodextrin solution having 1% (w/v) concentration by using the immobilized ANAG. Maltodextrin was completely converted to glucose after four hours. Consequently, it can be said that the immobilized ANAG obtained in this study can be used in the industrial production of glucose syrup.

Original languageEnglish
Pages (from-to)222-231
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume142
DOIs
Publication statusPublished - 1 Jan 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 Elsevier B.V.

Funding

The authors acknowledge to Bio-Cat Company for their kindly gift amyloglucosidase. The authors also acknowledge to Scientific Research Projects Coordinatorship of Siirt University for their financial support for the project encoded by 2017-SİÜFEB-28.

FundersFunder number
Siirt University2017-SİÜFEB-28

    Keywords

    • Activated carbon
    • Amyloglucosidase
    • Covalent immobilization

    Fingerprint

    Dive into the research topics of 'Covalent immobilization of Aspergillus niger amyloglucosidase (ANAG) with ethylenediamine-functionalized and glutaraldehyde-activated active carbon (EFGAAC) obtained from sesame seed shell'. Together they form a unique fingerprint.

    Cite this