Computational fluid dynamics modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering

Mahmut N. Cinbiz; R. Seda Tiǧli; Işil Gerçek Beşkardeş; Menemşe Gümüşderelioglu; Üner Çolak

doi:10.1016/j.jbiotec.2010.09.950

Computational fluid dynamics modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering

Mahmut N. Cinbiz, R. Seda Tiǧli, Işil Gerçek Beşkardeş, Menemşe Gümüşderelioglu^*, Üner Çolak

^*Corresponding author for this work

Hacettepe University

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

In this study, computational fluid dynamics (CFD) analysis of a rotating-wall perfused-vessel (RWPV) bioreactor is performed to characterize the complex hydrodynamic environment for the simulation of cartilage development in RWPV bioreactor in the presence of tissue-engineered cartilage constructs, i.e., cell-chitosan scaffolds. Shear stress exerted on chitosan scaffolds in bioreactor was calculated for different rotational velocities in the range of 33-38rpm. According to the calculations, the lateral and lower surfaces were exposed to 0.07926-0.11069dyne/cm² and 0.05974-0.08345dyne/cm², respectively, while upper surfaces of constructs were exposed to 0.09196-0.12847dyne/cm². Results validate adequate hydrodynamic environment for scaffolds in RWPV bioreactor for cartilage tissue development which concludes the suitability of operational conditions of RWPV bioreactor.

Original language	English
Pages (from-to)	389-395
Number of pages	7
Journal	Journal of Biotechnology
Volume	150
Issue number	3
DOIs	https://doi.org/10.1016/j.jbiotec.2010.09.950
Publication status	Published - Nov 2010
Externally published	Yes

Funding

This study was financially supported by Turkish Scientific and Research Council (Tübitak), Project No. 105M097.

Funders	Funder number
Turkish Scientific and Research Council	105M097

Keywords

Cartilage
Computational fluid dynamics
Rotating wall perfused vessel bioreactor
Tissue engineering

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10.1016/j.jbiotec.2010.09.950

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title = "Computational fluid dynamics modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering",

abstract = "In this study, computational fluid dynamics (CFD) analysis of a rotating-wall perfused-vessel (RWPV) bioreactor is performed to characterize the complex hydrodynamic environment for the simulation of cartilage development in RWPV bioreactor in the presence of tissue-engineered cartilage constructs, i.e., cell-chitosan scaffolds. Shear stress exerted on chitosan scaffolds in bioreactor was calculated for different rotational velocities in the range of 33-38rpm. According to the calculations, the lateral and lower surfaces were exposed to 0.07926-0.11069dyne/cm2 and 0.05974-0.08345dyne/cm2, respectively, while upper surfaces of constructs were exposed to 0.09196-0.12847dyne/cm2. Results validate adequate hydrodynamic environment for scaffolds in RWPV bioreactor for cartilage tissue development which concludes the suitability of operational conditions of RWPV bioreactor.",

keywords = "Cartilage, Computational fluid dynamics, Rotating wall perfused vessel bioreactor, Tissue engineering",

author = "Cinbiz, {Mahmut N.} and Tiǧli, {R. Seda} and Be{\c s}karde{\c s}, {I{\c s}il Ger{\c c}ek} and Menem{\c s}e G{\"u}m{\"u}{\c s}derelioglu and {\"U}ner {\c C}olak",

year = "2010",

month = nov,

doi = "10.1016/j.jbiotec.2010.09.950",

language = "English",

volume = "150",

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journal = "Journal of Biotechnology",

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AU - Cinbiz, Mahmut N.

AU - Tiǧli, R. Seda

AU - Beşkardeş, Işil Gerçek

AU - Gümüşderelioglu, Menemşe

AU - Çolak, Üner

PY - 2010/11

Y1 - 2010/11

N2 - In this study, computational fluid dynamics (CFD) analysis of a rotating-wall perfused-vessel (RWPV) bioreactor is performed to characterize the complex hydrodynamic environment for the simulation of cartilage development in RWPV bioreactor in the presence of tissue-engineered cartilage constructs, i.e., cell-chitosan scaffolds. Shear stress exerted on chitosan scaffolds in bioreactor was calculated for different rotational velocities in the range of 33-38rpm. According to the calculations, the lateral and lower surfaces were exposed to 0.07926-0.11069dyne/cm2 and 0.05974-0.08345dyne/cm2, respectively, while upper surfaces of constructs were exposed to 0.09196-0.12847dyne/cm2. Results validate adequate hydrodynamic environment for scaffolds in RWPV bioreactor for cartilage tissue development which concludes the suitability of operational conditions of RWPV bioreactor.

AB - In this study, computational fluid dynamics (CFD) analysis of a rotating-wall perfused-vessel (RWPV) bioreactor is performed to characterize the complex hydrodynamic environment for the simulation of cartilage development in RWPV bioreactor in the presence of tissue-engineered cartilage constructs, i.e., cell-chitosan scaffolds. Shear stress exerted on chitosan scaffolds in bioreactor was calculated for different rotational velocities in the range of 33-38rpm. According to the calculations, the lateral and lower surfaces were exposed to 0.07926-0.11069dyne/cm2 and 0.05974-0.08345dyne/cm2, respectively, while upper surfaces of constructs were exposed to 0.09196-0.12847dyne/cm2. Results validate adequate hydrodynamic environment for scaffolds in RWPV bioreactor for cartilage tissue development which concludes the suitability of operational conditions of RWPV bioreactor.

KW - Cartilage

KW - Computational fluid dynamics

KW - Rotating wall perfused vessel bioreactor

KW - Tissue engineering

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ER -

Computational fluid dynamics modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering

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