Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production

Abdulmecit Gökçe; Yavuz Öztürk; Zeynep Petek Çakar

doi:10.1016/j.ijhydene.2017.07.005

Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production

Abdulmecit Gökçe, Yavuz Öztürk, Zeynep Petek Çakar^*

^*Corresponding author for this work

Department of Molecular Biology and Genetics

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

To overcome efficiency losses due to overheating in outdoor photobioreactors for microbial hydrogen production, heat-resistant microorganisms are desirable. In this study, the comparative whole genome sequencing analyses of two previously obtained Rhodobacter capsulatus heat-resistant mutants; A52 and B41, with modified hydrogen production capacities, have been performed to identify mutations related to hydrogen production. In comparison with the reference strain DSM1710, the genomes of the mutants A52 and B41 contained 2137 and 2253 mutations, respectively. In the mutant B41 genome, mutations were characterized within the nifD, nifJ, glnD, nifB1, ccpA, hupD, dmsA, and cbbR1 genes. On the other hand; in the A52 mutant, mutations were characterized in the nifB2, rnfF, nifJ, cbbO, anfH, amt, moeA, and hupD genes. The effects of nitrogen metabolism and redox-related mutations were tested by quantitative reverse transcription PCR. The most promising mutation was found on the glnD gene of B41 strain with higher hydrogen production capacity.

Original language	English
Pages (from-to)	20529-20539
Number of pages	11
Journal	International Journal of Hydrogen Energy
Volume	42
Issue number	32
DOIs	https://doi.org/10.1016/j.ijhydene.2017.07.005
Publication status	Published - 10 Aug 2017

Bibliographical note

Publisher Copyright:
© 2017 Hydrogen Energy Publications LLC

Funding

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) [grant number 214Z138 (PI: Z. P. Cakar)]; Istanbul Technical University Research Funds for Ph.D. Thesis Projects [grant number 39572 (PI: Z. P. Cakar)]. Authors express their thanks to Bekir Ergüner, National Research Institute of Electronics and Cryptology, Advanced Genome & Bioinformatics Research Laboratory (TUBITAK), for the sequencing analyses.

Funders	Funder number
Advanced Genome & Bioinformatics Research Laboratory
National Research Institute of Electronics and Cryptology
Türkiye Bilimsel ve Teknolojik Araştirma Kurumu	214Z138
Istanbul Teknik Üniversitesi	39572

Keywords

Bacterial genomics
Biohydrogen
Next generation sequencing
PII uridylyl-transferase
Purple nonsulfur bacteria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ijhydene.2017.07.005

Cite this

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title = "Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production",

abstract = "To overcome efficiency losses due to overheating in outdoor photobioreactors for microbial hydrogen production, heat-resistant microorganisms are desirable. In this study, the comparative whole genome sequencing analyses of two previously obtained Rhodobacter capsulatus heat-resistant mutants; A52 and B41, with modified hydrogen production capacities, have been performed to identify mutations related to hydrogen production. In comparison with the reference strain DSM1710, the genomes of the mutants A52 and B41 contained 2137 and 2253 mutations, respectively. In the mutant B41 genome, mutations were characterized within the nifD, nifJ, glnD, nifB1, ccpA, hupD, dmsA, and cbbR1 genes. On the other hand; in the A52 mutant, mutations were characterized in the nifB2, rnfF, nifJ, cbbO, anfH, amt, moeA, and hupD genes. The effects of nitrogen metabolism and redox-related mutations were tested by quantitative reverse transcription PCR. The most promising mutation was found on the glnD gene of B41 strain with higher hydrogen production capacity.",

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author = "Abdulmecit G{\"o}k{\c c}e and Yavuz {\"O}zt{\"u}rk and {\c C}akar, {Zeynep Petek}",

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Comparative genomic analysis of two heat-resistant Rhodobacter capsulatus mutants with different hydrogen production levels reveals mutations related to hydrogen production. / Gökçe, Abdulmecit; Öztürk, Yavuz; Çakar, Zeynep Petek.
In: International Journal of Hydrogen Energy, Vol. 42, No. 32, 10.08.2017, p. 20529-20539.

Research output: Contribution to journal › Article › peer-review

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