Chronic impact of sulfamethoxazole on the metabolic activity and composition of enriched nitrifying microbial culture

Tugce Katipoglu-Yazan; Christophe Merlin; Marie Noëlle Pons; Emine Ubay-Cokgor; Derin Orhon

doi:10.1016/j.watres.2016.05.043

Chronic impact of sulfamethoxazole on the metabolic activity and composition of enriched nitrifying microbial culture

Tugce Katipoglu-Yazan^*, Christophe Merlin, Marie Noëlle Pons, Emine Ubay-Cokgor, Derin Orhon

^*Corresponding author for this work

Department of Environmental Engineering

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

This study investigated the chronic impact of sulfamethoxazole (SMX) on activated sludge sustaining an enriched nitrifying biomass. For this purpose, a laboratory scale fill and draw reactor was operated with 100 mg COD/L of peptone mixture and 50 mg N/L of ammonia at a sludge age of 15 days. Additionally, the biomass was exposed to a daily SMX dose of 50 mg/L once the reactor reached steady-state conditions. The reactor performance and microbial composition were monitored for 37 days with conventional parameters and molecular techniques based on the gene for ammonia monooxygenase subunit A (amoA) and the prokaryotic 16S rRNA gene. Denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene cloning analyses suggested a microbial community change concurrent with the addition of SMX. Specifically, quantitative polymerase chain reaction analyses (qPCR/RT-qPCR) revealed a significant reduction in the levels and activity of ammonia oxidizing bacteria (AOB). However, the acclimation period ended with high amoA mRNA levels and improved nitrification efficiency. Partial degradation of SMX by heterotrophic bacteria was also observed.

Original language	English
Pages (from-to)	546-555
Number of pages	10
Journal	Water Research
Volume	100
DOIs	https://doi.org/10.1016/j.watres.2016.05.043
Publication status	Published - 1 Sept 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd.

Funding

This study was conducted as a part of the project Evaluation of the Biodegradation Characteristics and Toxicity/Inhibition Effects of Selected Antibiotics on Nitrification Systems and supported by Scientific Research Fund of Istanbul Technical University. The authors would like to thank Dr. Xavier Bellanger and Hélène Guilloteau for their advice, assistance, and support throughout qPCR and cloning experiments. The authors also wish to thank Prof. G. Sockalingum and V. Untereiner for running the FT-IR analysis of EPS at the Faculty of Pharmacy, University of Reims-Champagne-Ardennes (France).

Funders	Funder number
Istanbul Teknik Üniversitesi

Keywords

Community molecular analyses
Enriched nitrifying culture
Gene activity
Nitrification
Sulfamethoxazole

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.watres.2016.05.043

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title = "Chronic impact of sulfamethoxazole on the metabolic activity and composition of enriched nitrifying microbial culture",

abstract = "This study investigated the chronic impact of sulfamethoxazole (SMX) on activated sludge sustaining an enriched nitrifying biomass. For this purpose, a laboratory scale fill and draw reactor was operated with 100 mg COD/L of peptone mixture and 50 mg N/L of ammonia at a sludge age of 15 days. Additionally, the biomass was exposed to a daily SMX dose of 50 mg/L once the reactor reached steady-state conditions. The reactor performance and microbial composition were monitored for 37 days with conventional parameters and molecular techniques based on the gene for ammonia monooxygenase subunit A (amoA) and the prokaryotic 16S rRNA gene. Denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene cloning analyses suggested a microbial community change concurrent with the addition of SMX. Specifically, quantitative polymerase chain reaction analyses (qPCR/RT-qPCR) revealed a significant reduction in the levels and activity of ammonia oxidizing bacteria (AOB). However, the acclimation period ended with high amoA mRNA levels and improved nitrification efficiency. Partial degradation of SMX by heterotrophic bacteria was also observed.",

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author = "Tugce Katipoglu-Yazan and Christophe Merlin and Pons, {Marie No{\"e}lle} and Emine Ubay-Cokgor and Derin Orhon",

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AU - Pons, Marie Noëlle

AU - Ubay-Cokgor, Emine

AU - Orhon, Derin

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AB - This study investigated the chronic impact of sulfamethoxazole (SMX) on activated sludge sustaining an enriched nitrifying biomass. For this purpose, a laboratory scale fill and draw reactor was operated with 100 mg COD/L of peptone mixture and 50 mg N/L of ammonia at a sludge age of 15 days. Additionally, the biomass was exposed to a daily SMX dose of 50 mg/L once the reactor reached steady-state conditions. The reactor performance and microbial composition were monitored for 37 days with conventional parameters and molecular techniques based on the gene for ammonia monooxygenase subunit A (amoA) and the prokaryotic 16S rRNA gene. Denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene cloning analyses suggested a microbial community change concurrent with the addition of SMX. Specifically, quantitative polymerase chain reaction analyses (qPCR/RT-qPCR) revealed a significant reduction in the levels and activity of ammonia oxidizing bacteria (AOB). However, the acclimation period ended with high amoA mRNA levels and improved nitrification efficiency. Partial degradation of SMX by heterotrophic bacteria was also observed.

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Chronic impact of sulfamethoxazole on the metabolic activity and composition of enriched nitrifying microbial culture

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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