Abstract
This study has aimed to evaluate comparative antibacterial activity of basil essential oil against clinical and standard isolates of Campylobacter spp. by different methods as agar well diffusion, agar and broth dilution methods. Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS) analysis were also examined to determine the chemical composition of the tested essential oil. GC/ MS analysis showed that, basil essential oil was predominated by methyl chavicol (86.6%) followed by 1,8-cineole (2.8%) and α-bergamotene (2.4%). Although, inhibition zone diameters were in the range of 10.9±0.8 to 21.8±1.4 mm, higher MIC values were obtained against clinical strains compared with standard ones. Due to the differences in antimicrobial resistance of the clinical and standard strains, antimicrobial activity tests should be carried out with isolates from different sources.
| Original language | English |
|---|---|
| Pages (from-to) | 183-191 |
| Number of pages | 9 |
| Journal | Acta Pharmaceutica Sciencia |
| Volume | 57 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2019 |
Bibliographical note
Publisher Copyright:© 2019, İstanbul Medipol University. All rights reserved.
Funding
The authors wish to thank Anadolu University, Medicinal Plants, Drugs and Scientific Research Center for GC and GC-MS analyses of basil essential oil. This research was supported by Istanbul Technical University, Scientific Research Projects (Project no, 38819).
| Funders | Funder number |
|---|---|
| Istanbul Teknik Üniversitesi | 38819 |
| Anadolu Üniversitesi |
Keywords
- Agar dilution
- Agar well diffusion
- Basil essential oil
- Brotmicrodilution
- Chemical composition