Chemical and molecular characterization of metabolites from Flavobacterium sp.

Vildan Enisoglu-Atalay, Belkis Atasever-Arslan, Bugra Yaman, Rumeysa Cebecioglu, Aykut Kul, Selma Ozilhan, Fatih Ozen, Tunc Cata*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

In this study, a Flavobacterium sp. is isolated from natural spring, and identified using molecular techniques. Extracellular and intracellular secondary metabolites are identified using solid phase microextraction gas chromatography-mass spectrometry and ultra performance liquid chromatography. Cytotoxic activity of the extracellular compounds produced by the Flavobacterium sp. and quercetin as the standard are measured using ECV304 human endothelial cells in vitro. Our results show that Flavobacterim sp. isolate has the highest percentage of similarity with Flavobacterium cheonhonense strain ARSA-15 (99%). Quercetin is detected as the major extracellular compound produced by the Flavobacterium sp. Methanol extract of Flavobacterium sp. resulted in a higher cell viability results when compared to DMSO extracts. Computational chemistry approach was used and it has been found that polar solvent (methanol) contributed to higher antioxidant activity. In conclusion, Flavobacterium sp. can be used to produce quercetin for industrial purposes.

Original languageEnglish
Article numbere0205817
JournalPLoS ONE
Volume13
Issue number10
DOIs
Publication statusPublished - Oct 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018 Enisoglu-Atalay et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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