Characterization of Anatolian honeys based on minerals, bioactive components and principal component analysis

Hakan Kaygusuz, Filiz Tezcan, F. Bedia Erim, Oktay Yildiz, Huseyin Sahin, Zehra Can, Sevgi Kolayli*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

85 Citations (Scopus)


Our aim is the characterization of Anatolian monofloral and honeydew honeys according to their mineral, vitamin B2, total phenolic contents and antioxidant activities. Five main elements (Ca, K, Fe, Cu, and Mn) were determined in 20 honey samples by inductively coupled plasma - optical emission spectrometry (ICP-OES). The vitamin B2 contents of honey samples were determined by the capillary electrophoresis method coupled with a sensitive laser induced fluorescence (LIF) detector. The total phenolic contents were analyzed with Folin-Ciocalteu's method. Two comparative antioxidant assays, namely cupric reducing antioxidant capacity assay (CUPRAC) and ABTS radical scavenging assay, were applied to detect the antioxidant power of honeys. Heather honeys were distinguished from others with significantly high vitamin B2 and iron contents. Considerably higher antioxidant capacities and Mn contents were observed for oak and chestnut honeys. Principal components analysis was applied to the analysis result in order to classify the honeys from different botanical origins.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
Publication statusPublished - 1 May 2016

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd.


This study was supported by TUBİTAK project ( 114Z370 ). The authors are also would like to thank experienced beekeepers Mr. Zekeriya Aydın, Yusuf Külekçi, Rıdvan Ulus, Ziya Şahin and Necati Aydın, who supplied the honey samples.

FundersFunder number


    • Antioxidant
    • Heather honey
    • Honey
    • Minerals
    • Vitamin B2


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