TY - JOUR
T1 - Characteristics of mixed microbial culture at different sludge ages
T2 - Effect on variable kinetics for substrate utilization
AU - Pala-Ozkok, Ilke
AU - Rehman, Ateequr
AU - Yagci, Nevin
AU - Ubay-Cokgor, Emine
AU - Jonas, Daniel
AU - Orhon, Derin
N1 - Publisher Copyright:
© 2012 Elsevier Ltd
PY - 2012
Y1 - 2012
N2 - The study focused on variable kinetics for substrate utilization, primarily addressing the following issue: Is variable process kinetics observed under different operating conditions and culture history (sludge ages), the result of changes inflicted on the metabolic machinery of the same microbial culture? Or, is this the result of a different microbial population selected under different operating conditions? For this purpose, the study mainly emphasized to assess the microbial population composition sustained at different sludge ages. It explored the relationship between observed process kinetics and microbial population structure using respirometric modeling and high-throughput sequencing of 16S rRNA gene. Experimental results indicated a significant change in the composition of the microbial community fed with the same organic substrate, when the culture history was changed, lower sludge age selecting a different and faster growing microbial community.
AB - The study focused on variable kinetics for substrate utilization, primarily addressing the following issue: Is variable process kinetics observed under different operating conditions and culture history (sludge ages), the result of changes inflicted on the metabolic machinery of the same microbial culture? Or, is this the result of a different microbial population selected under different operating conditions? For this purpose, the study mainly emphasized to assess the microbial population composition sustained at different sludge ages. It explored the relationship between observed process kinetics and microbial population structure using respirometric modeling and high-throughput sequencing of 16S rRNA gene. Experimental results indicated a significant change in the composition of the microbial community fed with the same organic substrate, when the culture history was changed, lower sludge age selecting a different and faster growing microbial community.
KW - Intracellular storage
KW - Microbial population
KW - Modeling
KW - Pyrosequencing
KW - Respirometry
UR - http://www.scopus.com/inward/record.url?scp=84867380167&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2012.08.115
DO - 10.1016/j.biortech.2012.08.115
M3 - Article
C2 - 23079414
AN - SCOPUS:84867380167
SN - 0960-8524
VL - 126
SP - 274
EP - 282
JO - Bioresource Technology
JF - Bioresource Technology
ER -