Characteristics of mixed microbial culture at different sludge ages: Effect on variable kinetics for substrate utilization

Ilke Pala-Ozkok*, Ateequr Rehman, Nevin Yagci, Emine Ubay-Cokgor, Daniel Jonas, Derin Orhon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

The study focused on variable kinetics for substrate utilization, primarily addressing the following issue: Is variable process kinetics observed under different operating conditions and culture history (sludge ages), the result of changes inflicted on the metabolic machinery of the same microbial culture? Or, is this the result of a different microbial population selected under different operating conditions? For this purpose, the study mainly emphasized to assess the microbial population composition sustained at different sludge ages. It explored the relationship between observed process kinetics and microbial population structure using respirometric modeling and high-throughput sequencing of 16S rRNA gene. Experimental results indicated a significant change in the composition of the microbial community fed with the same organic substrate, when the culture history was changed, lower sludge age selecting a different and faster growing microbial community.

Original languageEnglish
Pages (from-to)274-282
Number of pages9
JournalBioresource Technology
Volume126
DOIs
Publication statusPublished - 2012

Bibliographical note

Publisher Copyright:
© 2012 Elsevier Ltd

Keywords

  • Intracellular storage
  • Microbial population
  • Modeling
  • Pyrosequencing
  • Respirometry

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