Abstract
aveolae appear in a multitude of processes encompassing growth regulation and trafficking. We demonstrate the abundant presence of ESA/reggie-1/flotillin- 2, ATP synthase β subunit and annexin V in endothelial caveolae by immunopurification of caveolae from vascular endothelial membrane. Five proteins are abundant in a caveolin-1 protein complex, analyzed by sucrose gradient velocity sedimentation following octyl-β-D-glucopyranoside extraction. Caveolin-1 α interacts with caveolin-1 β, caveolin-2, actin, the microsomal form of NADH cytochrome B5 reductase and ESA/reggie-1/flotillin-2 as shown by co-immunoprecipitation. We propose the concept that ATP biosynthesis in caveolae regulates mechanosignaling and is induced by membrane depolarization and a proton gradient. Pressure stimuli and metabolic changes may trigger gene regulation in endothelial cells, involving a nuclear conformer of caveolin-1, shown here with an epitope-specific caveolin-1 antibody, and immediate response of ion channel activity, regulated by ESA/reggie-1/flotillin-2.
| Original language | English |
|---|---|
| Pages (from-to) | 67-84 |
| Number of pages | 18 |
| Journal | Molecular Biology Reports |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 2004 |
| Externally published | Yes |
Funding
We thank Thierry Mini for help with mass spectrometric analysis and Helena Bühler, Joachim Scherf and Kitaru Suda for technical support and Nica Borgese for antibodies. The Swiss National Science Foundation and Horten Foundation is thanked for financial support.
| Funders | Funder number |
|---|---|
| Horten Foundation | |
| Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung |