Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection

Jennifer H. Cheeseman; Michael G. Kaiser; Ceren Ciraci; Pete Kaiser; Susan J. Lamont

doi:10.1016/j.dci.2006.04.001

Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection

Jennifer H. Cheeseman, Michael G. Kaiser, Ceren Ciraci, Pete Kaiser, Susan J. Lamont^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

89 Citations (Scopus)

Abstract

We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-γ, Interleukin [IL]-1β, IL-6, IL-10, IL-12α, IL-12β, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-γ expression. Breed effect was significant (P < 0.05) for CXCLi2, IL-10, IL-12α, and CCLi2 mRNA expression in the spleen, and for IL-12α, IL-12β, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds.

Original language	English
Pages (from-to)	52-60
Number of pages	9
Journal	Developmental and Comparative Immunology
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.dci.2006.04.001
Publication status	Published - 2007
Externally published	Yes

Funding

The authors thank Jason Hasenstein and Huaijun Zhou for excellent technical assistance and sample preparation. This work was supported by National Research Initiative Grant no. 2004-35205-14234 from the USDA Cooperative State Research, Education and Extension Service; Research Grant IS-3021-98CR from BARD, the Binational Agriculture Research and Development Fund; and Animal Health, State of Iowa and the Iowa State University Center for Integrated Animal Genomics Funds.

Funders	Funder number
Animal Health, State of Iowa
Binational Agriculture Research and Development Fund
Cooperative State Research, Education, and Extension Service	IS-3021-98CR
BARD

Keywords

Cecum
Chicken
Cytokines
Genetics
Immunology
Salmonella enteritidis
Spleen

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10.1016/j.dci.2006.04.001

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title = "Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection",

abstract = "We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-γ, Interleukin [IL]-1β, IL-6, IL-10, IL-12α, IL-12β, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-γ expression. Breed effect was significant (P < 0.05) for CXCLi2, IL-10, IL-12α, and CCLi2 mRNA expression in the spleen, and for IL-12α, IL-12β, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds.",

keywords = "Cecum, Chicken, Cytokines, Genetics, Immunology, Salmonella enteritidis, Spleen",

author = "Cheeseman, {Jennifer H.} and Kaiser, {Michael G.} and Ceren Ciraci and Pete Kaiser and Lamont, {Susan J.}",

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AU - Cheeseman, Jennifer H.

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AU - Ciraci, Ceren

AU - Kaiser, Pete

AU - Lamont, Susan J.

PY - 2007

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AB - We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-γ, Interleukin [IL]-1β, IL-6, IL-10, IL-12α, IL-12β, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-γ expression. Breed effect was significant (P < 0.05) for CXCLi2, IL-10, IL-12α, and CCLi2 mRNA expression in the spleen, and for IL-12α, IL-12β, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds.

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Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection

Abstract

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