Biological control of green mould on mandarin fruit through the combined use of antagonistic yeasts

Sebahat Öztekin, Funda Karbancioglu-Guler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Penicillium digitatum is the primary cause of green mould in mandarins during the postharvest period, mainly controlled through synthetic fungicides. This study evaluated the biocontrol of green mould on mandarin fruit with three antagonistic yeasts (Hanseniaspora uvarum, Meyerozyma guilliermondii, and Metschnikowia aff. pulcherrima P01A016), alone or in combination, by elucidating their possible mechanisms of action. All yeast cultures alone (73.85 % − 80.64 %) and their combinations (78.40 % − 83.18 %) reduced the mycelial growth of green mold in vitro. M. aff. pulcherrima reduced the disease incidence and lesion diameters by 75.5 % and 91.3 %, respectively, demonstrating the highest biocontrolling activity alone. Meyerozyma guilliermondii showed the highest biofilm formation (OD 0.93 ± 0.01) and antifungal activity (71.13 %) through volatile organic compounds (VOCs), while H. uvarum displayed cell-wall degrading β-1,3 glucanase activity together with mycocinogenic and VOCs activity. All tested yeasts showed chitinase, protease, and leucine arylamidase activity. Among dual combinations, M. guilliermondii and M. aff. pulcherrima (M-1) were the most compatible, while M. aff. pulcherrima and H. uvarum (M-3) had the least compatibility. The combined application of these three yeasts resulted in synergistic co-operation, demonstrating the highest biocontrolling efficacy in vitro and in vivo. Integrating multiple mechanisms of action in yeasts could create a hurdle approach for inhibiting green mould in citrus as a green alternative to synthetic fungicides. Thus, our study demonstrated that combining biocontrol yeasts can result in beneficial consortia for the eco-friendly and sustainable management of P.digitatum-caused postharvest diseases of mandarins in both the wounded and intact mandarin fruits.

Original languageEnglish
Article number105186
JournalBiological Control
Volume180
DOIs
Publication statusPublished - May 2023

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Inc.

Funding

This work was financially supported by the Scientific Research Council of Istanbul Technical University (Grant no: MDK-2018-40878). The authors would like to express their gratitude to Dr. Bilal Ağırman (Çukurova University, Department of Food Engineering, Adana, Turkey) and Prof. Dr. Yeşim Özbaş (Hacettepe University, Department of Food Engineering, Ankara, Turkey), who kindly shared their culture collection to supply Penicillium digitatum DSM2750 and Saccharomyces cerevisiae NCYC 1006, respectively. This work was financially supported by the Scientific Research Council of Istanbul Technical University (Grant no: MDK-2018-40878 ). The authors would like to express their gratitude to Dr. Bilal Ağırman (Çukurova University, Department of Food Engineering, Adana, Turkey) and Prof. Dr. Yeşim Özbaş (Hacettepe University, Department of Food Engineering, Ankara, Turkey), who kindly shared their culture collection to supply Penicillium digitatum DSM2750 and Saccharomyces cerevisiae NCYC 1006, respectively.

FundersFunder number
Hacettepe ÜniversitesiDSM2750
Istanbul Teknik ÜniversitesiMDK-2018-40878

    Keywords

    • Biofungicide
    • Biological control
    • Citrus
    • Mycocinogenic activity
    • Penicillium digitatum
    • Postharvest

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