Bioaugmentation of sequencing batch reactors for biological phosphorus removal: Comparative rRNA sequence analysis and hybridization with oligonucleotide probes

Daniel B. Oerther; James Danalewich; Ebru Dulekgurgen; Eric Leveque; David L. Freedman; Lutgarde Raskin

doi:10.1016/S0273-1223(98)00148-6

Bioaugmentation of sequencing batch reactors for biological phosphorus removal: Comparative rRNA sequence analysis and hybridization with oligonucleotide probes

Daniel B. Oerther^*
, James Danalewich
, Ebru Dulekgurgen
, Eric Leveque
, David L. Freedman
, Lutgarde Raskin

^*Corresponding author for this work

University of Illinois at Urbana-Champaign
Clemson University College of Engineering, Computing and Applied Sciences

Research output: Contribution to journal › Conference article › peer-review

23 Citations (Scopus)

Abstract

Four laboratory-scale sequencing batch reactors (SBRs) were operated to evaluate whether bioaugmentation with Acinetobacter spp. can be used to improve start-up and performance of enhanced biological phosphorus removal (EBPR) systems. Two of the SBRs were bioaugmented during start-up by adding pure cultures of Acinetobacter spp., the third reactor received an amendment of activated sludge from a laboratory-scale EBPR system, and the fourth reactor, receiving no amendment, served as a control. Various chemical parameters were measured to monitor the performance of the four SBRS. Oligonucleotide probes of nested phylogenetic specificity were designed to quantify the contribution of Acinetobacter to EBPR. The probes were characterized for use in quantitative membrane hybridizations and fluorescent in situ hybridizations. Data from hybridizations with samples collected from the SBRs show declining levels of Acinetobacter spp. over the experiment. All four reactors achieved significant phosphorus removal and 90% nitrification after three days of operation. The results do not show a positive correlation between levels of Acinetobacter and successful EBPR.

Original language	English
Pages (from-to)	469-473
Number of pages	5
Journal	Water Science and Technology
Volume	37
Issue number	4-5
DOIs	https://doi.org/10.1016/S0273-1223(98)00148-6
Publication status	Published - 1998
Externally published	Yes
Event	Proceedings of the 1997 2nd International Conference on Microorganisms in Activated Sludge and Biofilm Processes - Berkeley, CA, USA Duration: 21 Jul 1997 → 23 Jul 1997

Funding

We are thankful 10 Tim Bachman and the ~taff at the Urbana-Champaign Sanitary DistricI for their continued support. This research was supported by the U.S. Department of Agriculture (95-37500-1911) and Seghers Engineering Water (Wespelaar, Belgium). Funding for Ebru Dulekgurgen was generously proVided by the Turkish Government.

Funders	Funder number
Seghers Engineering Water
U.S. Department of Agriculture	95-37500-1911

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation
SDG 14 Life Below Water

Keywords

Acinetobacter
Bioaugmentation
EBPR
Enhanced biological phosphorus removal
Molecular microbial ecology
Oligonucleotide probes
Ribosomal RNA
Sequencing batch reactors
Start-up

Access to Document

10.1016/S0273-1223(98)00148-6

Cite this

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title = "Bioaugmentation of sequencing batch reactors for biological phosphorus removal: Comparative rRNA sequence analysis and hybridization with oligonucleotide probes",

abstract = "Four laboratory-scale sequencing batch reactors (SBRs) were operated to evaluate whether bioaugmentation with Acinetobacter spp. can be used to improve start-up and performance of enhanced biological phosphorus removal (EBPR) systems. Two of the SBRs were bioaugmented during start-up by adding pure cultures of Acinetobacter spp., the third reactor received an amendment of activated sludge from a laboratory-scale EBPR system, and the fourth reactor, receiving no amendment, served as a control. Various chemical parameters were measured to monitor the performance of the four SBRS. Oligonucleotide probes of nested phylogenetic specificity were designed to quantify the contribution of Acinetobacter to EBPR. The probes were characterized for use in quantitative membrane hybridizations and fluorescent in situ hybridizations. Data from hybridizations with samples collected from the SBRs show declining levels of Acinetobacter spp. over the experiment. All four reactors achieved significant phosphorus removal and 90\% nitrification after three days of operation. The results do not show a positive correlation between levels of Acinetobacter and successful EBPR.",

keywords = "Acinetobacter, Bioaugmentation, EBPR, Enhanced biological phosphorus removal, Molecular microbial ecology, Oligonucleotide probes, Ribosomal RNA, Sequencing batch reactors, Start-up",

author = "Oerther, \{Daniel B.\} and James Danalewich and Ebru Dulekgurgen and Eric Leveque and Freedman, \{David L.\} and Lutgarde Raskin",

year = "1998",

doi = "10.1016/S0273-1223(98)00148-6",

language = "English",

volume = "37",

pages = "469--473",

journal = "Water Science and Technology",

issn = "0273-1223",

number = "4-5",

note = "Proceedings of the 1997 2nd International Conference on Microorganisms in Activated Sludge and Biofilm Processes ; Conference date: 21-07-1997 Through 23-07-1997",

}

Bioaugmentation of sequencing batch reactors for biological phosphorus removal: Comparative rRNA sequence analysis and hybridization with oligonucleotide probes. / Oerther, Daniel B.; Danalewich, James; Dulekgurgen, Ebru et al.
In: Water Science and Technology, Vol. 37, No. 4-5, 1998, p. 469-473.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Bioaugmentation of sequencing batch reactors for biological phosphorus removal

T2 - Proceedings of the 1997 2nd International Conference on Microorganisms in Activated Sludge and Biofilm Processes

AU - Oerther, Daniel B.

AU - Danalewich, James

AU - Dulekgurgen, Ebru

AU - Leveque, Eric

AU - Freedman, David L.

AU - Raskin, Lutgarde

PY - 1998

Y1 - 1998

N2 - Four laboratory-scale sequencing batch reactors (SBRs) were operated to evaluate whether bioaugmentation with Acinetobacter spp. can be used to improve start-up and performance of enhanced biological phosphorus removal (EBPR) systems. Two of the SBRs were bioaugmented during start-up by adding pure cultures of Acinetobacter spp., the third reactor received an amendment of activated sludge from a laboratory-scale EBPR system, and the fourth reactor, receiving no amendment, served as a control. Various chemical parameters were measured to monitor the performance of the four SBRS. Oligonucleotide probes of nested phylogenetic specificity were designed to quantify the contribution of Acinetobacter to EBPR. The probes were characterized for use in quantitative membrane hybridizations and fluorescent in situ hybridizations. Data from hybridizations with samples collected from the SBRs show declining levels of Acinetobacter spp. over the experiment. All four reactors achieved significant phosphorus removal and 90% nitrification after three days of operation. The results do not show a positive correlation between levels of Acinetobacter and successful EBPR.

AB - Four laboratory-scale sequencing batch reactors (SBRs) were operated to evaluate whether bioaugmentation with Acinetobacter spp. can be used to improve start-up and performance of enhanced biological phosphorus removal (EBPR) systems. Two of the SBRs were bioaugmented during start-up by adding pure cultures of Acinetobacter spp., the third reactor received an amendment of activated sludge from a laboratory-scale EBPR system, and the fourth reactor, receiving no amendment, served as a control. Various chemical parameters were measured to monitor the performance of the four SBRS. Oligonucleotide probes of nested phylogenetic specificity were designed to quantify the contribution of Acinetobacter to EBPR. The probes were characterized for use in quantitative membrane hybridizations and fluorescent in situ hybridizations. Data from hybridizations with samples collected from the SBRs show declining levels of Acinetobacter spp. over the experiment. All four reactors achieved significant phosphorus removal and 90% nitrification after three days of operation. The results do not show a positive correlation between levels of Acinetobacter and successful EBPR.

KW - Acinetobacter

KW - Bioaugmentation

KW - EBPR

KW - Enhanced biological phosphorus removal

KW - Molecular microbial ecology

KW - Oligonucleotide probes

KW - Ribosomal RNA

KW - Sequencing batch reactors

KW - Start-up

UR - https://www.scopus.com/pages/publications/0031831328

U2 - 10.1016/S0273-1223(98)00148-6

DO - 10.1016/S0273-1223(98)00148-6

M3 - Conference article

AN - SCOPUS:0031831328

SN - 0273-1223

VL - 37

SP - 469

EP - 473

JO - Water Science and Technology

JF - Water Science and Technology

IS - 4-5

Y2 - 21 July 1997 through 23 July 1997

ER -

Bioaugmentation of sequencing batch reactors for biological phosphorus removal: Comparative rRNA sequence analysis and hybridization with oligonucleotide probes

Abstract

Funding

UN SDGs

Keywords

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