Author Correction: Effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate (Scientific Reports, (2020), 10, 1, (3510), 10.1038/s41598-020-60622-1)

Hande Ermis*, Unzile Guven-Gulhan, Tunahan Cakir, Mahmut Altinbas

*Corresponding author for this work

Research output: Contribution to journalComment/debate

Abstract

Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515 f (F), 5′-GTG CCA GCMGCC GCG GTAA-3′ and 806r (R), 5′-GGA CTA CHVHHHTWT CTA AT-3′] for 16S, TAReuk454FWD1 (F), 5′-CCA GCA SCYG CGG TAA TTC -3′ and TAReukREV3 (R), 5′-ACT TTC GTT CTT GAT YRA -3′ primers for 18S rDNA, p23SrV_f1 (F), 5′-GGA CAG AAA GAC CCT ATG AA-3′ and p23SrV_r1 (R), 5′-TCA GCC TGT-TAT CCC TAGAG-3′ primers for 23S rDNA, (F) 5′-TGA AAC AGAAMAWC GTC ATT-3′ and (R) 5′-CCTTCNCGAATMGCR AAW -3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).” should read: “PCR amplification and sequence analyses of 16S rRNA, 18S rRNA, 23S rRNA and tufa Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515f (F), 5′-GTG YCA GCMGCC GCG GTAA-3′1 and 806r (R), 5′-GGA CTA CNVGGG TWT CTAAT-3′2] for 16S, TAReuk454FWD1 (F), 5′-CCA GCA SCYG CGG TAA TTC -3′ and TAReukREV3 (R), 5′-ACT TTC GTT CTT GAT YRA -3′ primers3 for 18S rDNA, p23SrV_f1 (F), 5′-GGA CAG AAA GAC CCT ATG AA-3′ and p23SrV_r1 (R), 5′-TCA GCC TGT TAT CCC TAG AG-3′ primers4 for 23S rDNA, (F) 5′-TGA AAC AGAAMAWC GTC ATT-3′ and (R) 5′-CCTTCNCGAATMGCR AAW -3′ primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).” The omitted References are listed below as References 1–4 respectively.

Original languageEnglish
Article number11963
JournalScientific Reports
Volume10
Issue number1
DOIs
Publication statusPublished - 1 Dec 2020

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© 2020, The Author(s).

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