Assessment of anaerobic bacterial diversity and its effects on anaerobic system stability and the occurrence of antibiotic resistance genes

Sevcan Aydin*, Bahar Ince, Orhan Ince

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

This study evaluated the link between anaerobic bacterial diversity and, the biodegradation of antibiotic combinations and assessed how amending antibiotic combination and increasing concentration of antibiotics in a stepwise fashion influences the development of resistance genes in anaerobic reactors. The biodegradation, sorption and occurrence of the known antibiotic resistance genes (ARGs) of erythromycin and tetracycline were investigated using the processes of UV-HPLC and qPCR analysis respectively. Ion Torrent sequencing was used to detect microbial community changes in response to the addition of antibiotics. The overall results indicated that changes in the structure of a microbial community lead to changes in biodegradation capacity, sorption of antibiotics combinations and occurrence of ARGs. The enhanced biodegradation efficiency appeared to generate variations in the structure of the bacterial community. The results suggested that controlling the ultimate Gram-negative bacterial community, especially Acinetobacter-related populations, may promote the successful biodegradation of antibiotic combinations and reduce the occurrence of ARGs.

Original languageEnglish
Pages (from-to)332-338
Number of pages7
JournalBioresource Technology
Volume207
DOIs
Publication statusPublished - 1 May 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd.

Funding

The authors thank TUBITAK (Turkish Association of Science and Technology) for their support of this research (No: 110Y310).

FundersFunder number
TUBITAK
Turkish Association of Science and Technology110Y310

    Keywords

    • Anaerobic bacterial diversity
    • Antibiotic resistance
    • Antibiotic resistance genes
    • Antibiotics
    • Microbial community

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