Abstract
Archaeal diversity in Lake Aci{dotless}göl, a closed-basin, alkaline, hypersaline lake located at the northern edge of western Tourides in southwest Anatolia, was investigated using culture-independent methods. Microbial mat samples were collected from six different points. Archaeal 16S rRNA gene libraries were generated using domain specific oligonucleotide primers, and 16S rRNA gene sequences of clone libraries were analyzed phylogenetically. Denaturing gradient gel electrophoresis of 16S rRNA genes showed a variance in diversity with spatial differences. Archaeal diversity of Aci{dotless}göl is dominated by the members of family Halobacteriaceae which requires both high salt concentration and high pH for growth. Sequence analysis of archaeal 16s rRNA genes indicates the presence of the phylotypes affiliated with the genera Halorubrum, Halosimplex, Halorhabdus, Haloterrigena and Natronococcus in the analyzed samples.
| Original language | English |
|---|---|
| Pages (from-to) | 454-460 |
| Number of pages | 7 |
| Journal | Geomicrobiology Journal |
| Volume | 31 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jul 2014 |
Funding
This research was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) project.
| Funders | Funder number |
|---|---|
| TUBITAK | |
| Türkiye Bilimsel ve Teknolojik Araştirma Kurumu |
Keywords
- 16S rRNA
- Lake Aci{dotless}göl
- Soda Lake
- archaeal diversity
- halobacteria
- hypersaline
- phylogenetic aAnalysis