TY - JOUR
T1 - Archaeal Microbial Diversity of Hypersaline Lake Aci{dotless}göl, Denizli, Turkey
AU - Budakoglu, Murat
AU - Kurt, Halil
AU - Karaman, Muhittin
AU - Kumru, Mert
AU - Kumral, Mustafa
AU - Akarsubaşi, Alper Tunga
PY - 2014/7
Y1 - 2014/7
N2 - Archaeal diversity in Lake Aci{dotless}göl, a closed-basin, alkaline, hypersaline lake located at the northern edge of western Tourides in southwest Anatolia, was investigated using culture-independent methods. Microbial mat samples were collected from six different points. Archaeal 16S rRNA gene libraries were generated using domain specific oligonucleotide primers, and 16S rRNA gene sequences of clone libraries were analyzed phylogenetically. Denaturing gradient gel electrophoresis of 16S rRNA genes showed a variance in diversity with spatial differences. Archaeal diversity of Aci{dotless}göl is dominated by the members of family Halobacteriaceae which requires both high salt concentration and high pH for growth. Sequence analysis of archaeal 16s rRNA genes indicates the presence of the phylotypes affiliated with the genera Halorubrum, Halosimplex, Halorhabdus, Haloterrigena and Natronococcus in the analyzed samples.
AB - Archaeal diversity in Lake Aci{dotless}göl, a closed-basin, alkaline, hypersaline lake located at the northern edge of western Tourides in southwest Anatolia, was investigated using culture-independent methods. Microbial mat samples were collected from six different points. Archaeal 16S rRNA gene libraries were generated using domain specific oligonucleotide primers, and 16S rRNA gene sequences of clone libraries were analyzed phylogenetically. Denaturing gradient gel electrophoresis of 16S rRNA genes showed a variance in diversity with spatial differences. Archaeal diversity of Aci{dotless}göl is dominated by the members of family Halobacteriaceae which requires both high salt concentration and high pH for growth. Sequence analysis of archaeal 16s rRNA genes indicates the presence of the phylotypes affiliated with the genera Halorubrum, Halosimplex, Halorhabdus, Haloterrigena and Natronococcus in the analyzed samples.
KW - 16S rRNA
KW - Lake Aci{dotless}göl
KW - Soda Lake
KW - archaeal diversity
KW - halobacteria
KW - hypersaline
KW - phylogenetic aAnalysis
UR - http://www.scopus.com/inward/record.url?scp=84902655181&partnerID=8YFLogxK
U2 - 10.1080/01490451.2013.866994
DO - 10.1080/01490451.2013.866994
M3 - Article
AN - SCOPUS:84902655181
SN - 0149-0451
VL - 31
SP - 454
EP - 460
JO - Geomicrobiology Journal
JF - Geomicrobiology Journal
IS - 6
ER -
