Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids

F. B. Erim; X. Xu; J. C. Kraak

doi:10.1016/0021-9673(94)01114-T

Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids

F. B. Erim^*, X. Xu, J. C. Kraak

^*Corresponding author for this work

University of Amsterdam

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

A micellar electrokinetic chromatographic system using indirect UV detection was developed for the analysis of saturated fatty acids. Sodium dodecyl benzenesulfonate (SDBS) was used as micellar surfactant as well as chromophore for the indirect detection. The effects of the addition of acetonitrile and Brij on the migration and solubility of the fatty acids was investigated. With a mixture of 10 mM SDBS + 50% acetonitrile + 30 mM Brij, a complete separation of the C₈-C₂₀ fatty acids was obtained. The applicability of the developed system for the analysis of fatty acids in butter is demonstrated.

Original language	English
Pages (from-to)	471-479
Number of pages	9
Journal	Journal of Chromatography A
Volume	694
Issue number	2
DOIs	https://doi.org/10.1016/0021-9673(94)01114-T
Publication status	Published - 10 Mar 1995
Externally published	Yes

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10.1016/0021-9673(94)01114-T

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title = "Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids",

abstract = "A micellar electrokinetic chromatographic system using indirect UV detection was developed for the analysis of saturated fatty acids. Sodium dodecyl benzenesulfonate (SDBS) was used as micellar surfactant as well as chromophore for the indirect detection. The effects of the addition of acetonitrile and Brij on the migration and solubility of the fatty acids was investigated. With a mixture of 10 mM SDBS + 50% acetonitrile + 30 mM Brij, a complete separation of the C8-C20 fatty acids was obtained. The applicability of the developed system for the analysis of fatty acids in butter is demonstrated.",

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AU - Kraak, J. C.

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AB - A micellar electrokinetic chromatographic system using indirect UV detection was developed for the analysis of saturated fatty acids. Sodium dodecyl benzenesulfonate (SDBS) was used as micellar surfactant as well as chromophore for the indirect detection. The effects of the addition of acetonitrile and Brij on the migration and solubility of the fatty acids was investigated. With a mixture of 10 mM SDBS + 50% acetonitrile + 30 mM Brij, a complete separation of the C8-C20 fatty acids was obtained. The applicability of the developed system for the analysis of fatty acids in butter is demonstrated.

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ER -

Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids

Abstract

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