Antioxidant activity and capacity measurement

Esra Capanoglu, Senem Kamiloglu, Sema Demirci Cekic, Kevser Sozgen Baskan, Asli Neslihan Avan, Seda Uzunboy, Resat Apak

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Measurement of antioxidant activity/capacity of foods and biological fluids is important for the determination of nutritional value of foods and for the diagnosis of diseases associated with oxidative stress. Antioxidant activity/capacity can be monitored using assays with different mechanisms, including (i) electron transfer-based assays, (ii) hydrogen atom transfer-based assays, (iii) mixed-mode assays, (iv) lipid peroxidation assays, (v) ROS/RNS scavenging assays, (vi) oxidative stress biomarkers and cellular-based assays, and (vii) chromatographic, chemometric, and electrochemical assays. In this chapter, the mechanisms of the most widely used analytical approaches for the measurement of antioxidant activity/capacity are discussed together with their advantages and drawbacks. Overall, measurement of antioxidant activity/capacity, in particular for mixtures, multifunctional or complex multiphase systems, cannot be evaluated satisfactorily with a simple antioxidant assay due to the several variables affecting the results. Therefore, it is recommended to apply several assays with different mechanisms in order to reveal the full capability of an antioxidant sample.

Original languageEnglish
Pages (from-to)997-1027
Number of pages31
JournalReference Series in Phytochemistry
Issue number5
Publication statusPublished - 2021

Bibliographical note

Publisher Copyright:
© Springer Nature Switzerland AG 2021.


  • Antioxidant activity measurement
  • Cellular-based assays
  • Chemometric assays
  • Chromatographic assays
  • Electrochemical assays
  • Electron transfer-based assays
  • Hydrogen atom transfer-based assays
  • Lipid peroxidation assays
  • Mixed-mode assays
  • Oxidative stress biomarkers
  • ROS/RNS scavenging assays


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