TY - JOUR
T1 - Anti-inflammatory potential of black carrot (Daucus carota L.) polyphenols in a co-culture model of intestinal Caco-2 and endothelial EA.hy926 cells
AU - Kamiloglu, Senem
AU - Grootaert, Charlotte
AU - Capanoglu, Esra
AU - Ozkan, Ceren
AU - Smagghe, Guy
AU - Raes, Katleen
AU - Van Camp, John
N1 - Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Scope: The present study was developed to determine the ability of polyphenol-rich black carrot and its by-products, i.e., peel and pomace, to modulate the inflammatory response in tumor necrosis factor α (TNF-α) treated endothelial cells after gastrointestinal digestion and in a co-culture of intestinal Caco-2 and endothelial EA.hy926 cell model. Results: The results indicated that after 4 h of treatment, the transport of anthocyanins and phenolic acids was higher for digested samples (1.3−7%) compared to the undigested samples (0−3.3%). The transported polyphenols were able to downregulate the secretion of pro-inflammatory markers, i.e. IL-8, monocyte chemoattractant protein 1, vascular endothelial growth factor, and intercellular adhesion molecule 1, under normal and tumor necrosis factor α induced inflammatory conditions. The most pronounced protective effects were observed with digested samples under inflammatory conditions, which significantly decreased the secretion of all markers from 120−203% down to 34−144% (p < 0.001). Conclusions: Overall, these results show that the polyphenol-rich black carrot absorption products may function through an inhibitory regulation of the inflammatory cascade in endothelial cells.
AB - Scope: The present study was developed to determine the ability of polyphenol-rich black carrot and its by-products, i.e., peel and pomace, to modulate the inflammatory response in tumor necrosis factor α (TNF-α) treated endothelial cells after gastrointestinal digestion and in a co-culture of intestinal Caco-2 and endothelial EA.hy926 cell model. Results: The results indicated that after 4 h of treatment, the transport of anthocyanins and phenolic acids was higher for digested samples (1.3−7%) compared to the undigested samples (0−3.3%). The transported polyphenols were able to downregulate the secretion of pro-inflammatory markers, i.e. IL-8, monocyte chemoattractant protein 1, vascular endothelial growth factor, and intercellular adhesion molecule 1, under normal and tumor necrosis factor α induced inflammatory conditions. The most pronounced protective effects were observed with digested samples under inflammatory conditions, which significantly decreased the secretion of all markers from 120−203% down to 34−144% (p < 0.001). Conclusions: Overall, these results show that the polyphenol-rich black carrot absorption products may function through an inhibitory regulation of the inflammatory cascade in endothelial cells.
KW - Anthocyanins
KW - In vitro digestion
KW - Phenolic acids
KW - Pro-inflammatory markers
KW - Transepithelial transport
UR - http://www.scopus.com/inward/record.url?scp=85006804383&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201600455
DO - 10.1002/mnfr.201600455
M3 - Article
C2 - 27561918
AN - SCOPUS:85006804383
SN - 1613-4125
VL - 61
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 2
M1 - 1600455
ER -