Anodic Voltammetric Determination of an Atypical Antipsychotic Drug Amisulphide in Pharmaceutical Dosage Forms Using Electrochemical fsDNA Biosensor

Mustafa Cesme; Duygu Polat; Pelin Senel; Aysegul Golcu

doi:10.1134/S1023193519030042

Anodic Voltammetric Determination of an Atypical Antipsychotic Drug Amisulphide in Pharmaceutical Dosage Forms Using Electrochemical fsDNA Biosensor

Mustafa Cesme, Duygu Polat, Pelin Senel, Aysegul Golcu^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In this study, a new biosensor for amisulpride (ASP) determination is defined. The interaction between ASP and fish sperm double-stranded DNA (FSdsDNA) was examined by differential pulse voltammetry (DPV) method utilizing FSdsDNA modified pencil graphite electrode (PGE). Sensitive and reliable ASP determination and detection was performed in pH 4.80 acetate buffer (20% ethanol) before and after the interaction of ASP and FSdsDNA, acting by the change in guanine oxidation signals from the electro-active bases of FSdsDNA. In addition, analytical method validity tests were examined in this study. This modified electrode have used in 1–10 μg/mL determination interval for the quantitative determination of ASP in pharmaceutical forms and the limit of detection (LOD) has been found as 0.46 μg/mL. Also, the binding constants (K_b) of the ASP with FSdsDNA has also been calculated both UV-Vis and DPV techniques.

Original language	English
Pages (from-to)	281-290
Number of pages	10
Journal	Russian Journal of Electrochemistry
Volume	55
Issue number	4
DOIs	https://doi.org/10.1134/S1023193519030042
Publication status	Published - 1 Apr 2019

Bibliographical note

Publisher Copyright:
© 2019, Pleiades Publishing, Ltd.

Funding

FUNDING This work was supported by TUBITAK (Project no. 112T721) and Research Fund of the Kahramanmaras Sutcu Imam University (Project no. 2013/1-3 YLS).

Funders	Funder number
TUBITAK	112T721
Kahramanmaraş Sütçü Imam Üniversitesi	2013/1-3 YLS

Keywords

amisulpride
biosensor
determination
DPV
dsDNA
pencil graphite electrode
pharmaceuticals

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title = "Anodic Voltammetric Determination of an Atypical Antipsychotic Drug Amisulphide in Pharmaceutical Dosage Forms Using Electrochemical fsDNA Biosensor",

abstract = "In this study, a new biosensor for amisulpride (ASP) determination is defined. The interaction between ASP and fish sperm double-stranded DNA (FSdsDNA) was examined by differential pulse voltammetry (DPV) method utilizing FSdsDNA modified pencil graphite electrode (PGE). Sensitive and reliable ASP determination and detection was performed in pH 4.80 acetate buffer (20% ethanol) before and after the interaction of ASP and FSdsDNA, acting by the change in guanine oxidation signals from the electro-active bases of FSdsDNA. In addition, analytical method validity tests were examined in this study. This modified electrode have used in 1–10 μg/mL determination interval for the quantitative determination of ASP in pharmaceutical forms and the limit of detection (LOD) has been found as 0.46 μg/mL. Also, the binding constants (Kb) of the ASP with FSdsDNA has also been calculated both UV-Vis and DPV techniques.",

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AU - Cesme, Mustafa

AU - Polat, Duygu

AU - Senel, Pelin

AU - Golcu, Aysegul

PY - 2019/4/1

Y1 - 2019/4/1

N2 - In this study, a new biosensor for amisulpride (ASP) determination is defined. The interaction between ASP and fish sperm double-stranded DNA (FSdsDNA) was examined by differential pulse voltammetry (DPV) method utilizing FSdsDNA modified pencil graphite electrode (PGE). Sensitive and reliable ASP determination and detection was performed in pH 4.80 acetate buffer (20% ethanol) before and after the interaction of ASP and FSdsDNA, acting by the change in guanine oxidation signals from the electro-active bases of FSdsDNA. In addition, analytical method validity tests were examined in this study. This modified electrode have used in 1–10 μg/mL determination interval for the quantitative determination of ASP in pharmaceutical forms and the limit of detection (LOD) has been found as 0.46 μg/mL. Also, the binding constants (Kb) of the ASP with FSdsDNA has also been calculated both UV-Vis and DPV techniques.

AB - In this study, a new biosensor for amisulpride (ASP) determination is defined. The interaction between ASP and fish sperm double-stranded DNA (FSdsDNA) was examined by differential pulse voltammetry (DPV) method utilizing FSdsDNA modified pencil graphite electrode (PGE). Sensitive and reliable ASP determination and detection was performed in pH 4.80 acetate buffer (20% ethanol) before and after the interaction of ASP and FSdsDNA, acting by the change in guanine oxidation signals from the electro-active bases of FSdsDNA. In addition, analytical method validity tests were examined in this study. This modified electrode have used in 1–10 μg/mL determination interval for the quantitative determination of ASP in pharmaceutical forms and the limit of detection (LOD) has been found as 0.46 μg/mL. Also, the binding constants (Kb) of the ASP with FSdsDNA has also been calculated both UV-Vis and DPV techniques.

KW - amisulpride

KW - biosensor

KW - determination

KW - DPV

KW - dsDNA

KW - pencil graphite electrode

KW - pharmaceuticals

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DO - 10.1134/S1023193519030042

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JO - Russian Journal of Electrochemistry

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ER -

Anodic Voltammetric Determination of an Atypical Antipsychotic Drug Amisulphide in Pharmaceutical Dosage Forms Using Electrochemical fsDNA Biosensor

Abstract

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Keywords

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