Anodic voltammetric behavior and determination of cefixime in pharmaceutical dosage forms and biological fluids

Ayşegul Golcu; Burcu Dogan; Sibel A. Ozkan

doi:10.1016/j.talanta.2005.03.020

Anodic voltammetric behavior and determination of cefixime in pharmaceutical dosage forms and biological fluids

Ayşegul Golcu, Burcu Dogan, Sibel A. Ozkan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

85 Citations (Scopus)

Abstract

The voltammetric behavior of cefixime was studied using cyclic, linear sweep, differential pulse and square wave voltammetric techniques. The oxidation of cefixime was irreversible and exhibited diffusion controlled process depending on pH. The oxidation mechanism was proposed and discussed. Different parameters were tested to optimize the conditions for the determination of cefixime. The dependence of current intensities and potentials on pH, concentration, scan rate, nature of the buffer was investigated. According to the linear relationship between the peak current and the concentration, differential pulse (DPV) and square wave (SWV) voltammetric methods for cefixime assay in pharmaceutical dosage forms and biological fluids were developed. For the determination of cefixime were proposed in acetate buffer at pH 4.5, which allows quantitation over the 6 × 10^-6-2 × 10^-4 M range in supporting electrolyte and spiked serum sample; 8 × 10 ^-6-2 × 10^-4 M range in urine sample; 6 × 10^-6-1 × 10^-4 M range in breast milk samples for both techniques. The repeatability, reproducibility, precision and accuracy of the methods in all media were investigated. No electroactive interferences from the excipients and endogenous substances were found in the pharmaceutical dosage forms and in the biological samples, respectively.

Original language	English
Pages (from-to)	703-712
Number of pages	10
Journal	Talanta
Volume	67
Issue number	4
DOIs	https://doi.org/10.1016/j.talanta.2005.03.020
Publication status	Published - 15 Oct 2005
Externally published	Yes

Funding

The authors gratefully acknowledge Eczacıbası Pharm. Ind. for supplying cefixime standard sample and its pharmaceutical dosage forms. This work was realized the BAS 100W equipment which is supplied from Ankara University Scientific Research Foundation Project (Grant No: 20030803037).

Funders	Funder number
Ankara University Scientific Research Foundation Project	20030803037

Keywords

Breast milk samples
Cefixime
Oxidation
Pharmaceuticals
Serum samples
Urine samples
Voltammetry

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10.1016/j.talanta.2005.03.020

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abstract = "The voltammetric behavior of cefixime was studied using cyclic, linear sweep, differential pulse and square wave voltammetric techniques. The oxidation of cefixime was irreversible and exhibited diffusion controlled process depending on pH. The oxidation mechanism was proposed and discussed. Different parameters were tested to optimize the conditions for the determination of cefixime. The dependence of current intensities and potentials on pH, concentration, scan rate, nature of the buffer was investigated. According to the linear relationship between the peak current and the concentration, differential pulse (DPV) and square wave (SWV) voltammetric methods for cefixime assay in pharmaceutical dosage forms and biological fluids were developed. For the determination of cefixime were proposed in acetate buffer at pH 4.5, which allows quantitation over the 6 × 10-6-2 × 10-4 M range in supporting electrolyte and spiked serum sample; 8 × 10 -6-2 × 10-4 M range in urine sample; 6 × 10-6-1 × 10-4 M range in breast milk samples for both techniques. The repeatability, reproducibility, precision and accuracy of the methods in all media were investigated. No electroactive interferences from the excipients and endogenous substances were found in the pharmaceutical dosage forms and in the biological samples, respectively.",

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AB - The voltammetric behavior of cefixime was studied using cyclic, linear sweep, differential pulse and square wave voltammetric techniques. The oxidation of cefixime was irreversible and exhibited diffusion controlled process depending on pH. The oxidation mechanism was proposed and discussed. Different parameters were tested to optimize the conditions for the determination of cefixime. The dependence of current intensities and potentials on pH, concentration, scan rate, nature of the buffer was investigated. According to the linear relationship between the peak current and the concentration, differential pulse (DPV) and square wave (SWV) voltammetric methods for cefixime assay in pharmaceutical dosage forms and biological fluids were developed. For the determination of cefixime were proposed in acetate buffer at pH 4.5, which allows quantitation over the 6 × 10-6-2 × 10-4 M range in supporting electrolyte and spiked serum sample; 8 × 10 -6-2 × 10-4 M range in urine sample; 6 × 10-6-1 × 10-4 M range in breast milk samples for both techniques. The repeatability, reproducibility, precision and accuracy of the methods in all media were investigated. No electroactive interferences from the excipients and endogenous substances were found in the pharmaceutical dosage forms and in the biological samples, respectively.

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Anodic voltammetric behavior and determination of cefixime in pharmaceutical dosage forms and biological fluids

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