Anodic oxidation of antibacterial drug cefotaxime sodium and its square wave and differential pulsevoltammetric determination in pharmaceuticals and human serum

Burcu Dogan, Aysegul Golcu, Mustafa Dolaz, Sibel A. Ozkan*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

19 Citations (Scopus)

Abstract

Cefotaxime sodium has an antibacterial effect and it is classified as a third-generation broad spectrum cephalosporin antibiotic. It is irreversible, oxidized at the glassy carbon electrode in various buffer systems and at different pH values. The detailed mechanism of the oxidation process is described in this manuscript. Differential pulse and square wave voltammetric methods were developed for its determination in pharmaceutical dosage forms and spiked human serum samples according to the linear relation between the peak current and cefotaxime sodium concentration. For analytical purposes, a very well resolved diffusion controlled voltammetric peak was obtained in Britton-Robinson buffer at pH 2.0 at 0.87 and 0.89V for differential pulse and square wave voltammetric techniques, respectively. The linear response was obtained within the range of 1×10-6 - 6×10-5 M with a detection limit of 2.83×10-7 M for differential pulse and 2×10-6 - 6×10-5 M with a detection limit of 3.61×10-7 M for square wave voltammetric techniques. The repeatability and reproducibility of the methods for both media (supporting electrolyte and serum sample) were determined. Precision and accuracy of the developed method were used for the recovery studies. The standard addition method was used for the recovery studies. No electroactive interferences were found in biological fluids from the endogenous substances and additives present in pharmaceutical dosage form.

Original languageEnglish
Pages (from-to)197-207
Number of pages11
JournalCurrent Pharmaceutical Analysis
Volume5
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Biological samples
  • Cefotaxime
  • Determination
  • Oxidation
  • Pharmaceuticals
  • Voltammetry

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