An LED-based super resolution GPU implemented structured illumination microscope

Musa Aydin; Yiäit Uysalll; Ekin Özgönül; Berna Morova; Alper Kiraz

doi:10.1117/12.2544602

An LED-based super resolution GPU implemented structured illumination microscope

Musa Aydin, Yiäit Uysalll, Ekin Özgönül, Berna Morova, Alper Kiraz^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel-lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of â1/4150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.

Original language	English
Title of host publication	Single Molecule Spectroscopy and Superresolution Imaging XIII
Editors	Ingo Gregor, Felix Koberling, Rainer Erdmann
Publisher	SPIE
ISBN (Electronic)	9781510632554
DOIs	https://doi.org/10.1117/12.2544602
Publication status	Published - 2020
Externally published	Yes
Event	Single Molecule Spectroscopy and Superresolution Imaging XIII 2020 - San Francisco, United States Duration: 1 Feb 2020 → 2 Feb 2020

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11246
ISSN (Print)	1605-7422

Conference

Conference	Single Molecule Spectroscopy and Superresolution Imaging XIII 2020
Country/Territory	United States
City	San Francisco
Period	1/02/20 → 2/02/20

Bibliographical note

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Keywords

DMD
GPU
Structured Illumination Microscopy (SIM)
Super Resolution Microscopy

Access to Document

10.1117/12.2544602

Cite this

Aydin, M., Uysalll, Y., Özgönül, E., Morova, B., & Kiraz, A. (2020). An LED-based super resolution GPU implemented structured illumination microscope. In I. Gregor, F. Koberling, & R. Erdmann (Eds.), Single Molecule Spectroscopy and Superresolution Imaging XIII Article 1124610 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11246). SPIE. https://doi.org/10.1117/12.2544602

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abstract = "Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel-lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of {\^a}1/4150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.",

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doi = "10.1117/12.2544602",

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Aydin, M, Uysalll, Y, Özgönül, E, Morova, B & Kiraz, A 2020, An LED-based super resolution GPU implemented structured illumination microscope. in I Gregor, F Koberling & R Erdmann (eds), Single Molecule Spectroscopy and Superresolution Imaging XIII., 1124610, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11246, SPIE, Single Molecule Spectroscopy and Superresolution Imaging XIII 2020, San Francisco, United States, 1/02/20. https://doi.org/10.1117/12.2544602

An LED-based super resolution GPU implemented structured illumination microscope. / Aydin, Musa; Uysalll, Yiäit; Özgönül, Ekin et al.
Single Molecule Spectroscopy and Superresolution Imaging XIII. ed. / Ingo Gregor; Felix Koberling; Rainer Erdmann. SPIE, 2020. 1124610 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11246).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - An LED-based super resolution GPU implemented structured illumination microscope

AU - Aydin, Musa

AU - Uysalll, Yiäit

AU - Özgönül, Ekin

AU - Morova, Berna

AU - Kiraz, Alper

N1 - Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2020

Y1 - 2020

N2 - Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel-lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of â1/4150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.

AB - Fluorescence imaging of sub-cellular structures with sizes below the diffraction limit is vital in understanding cel-lular processes. Relying on exciting the sample with different illumination patterns and image processing for the elimination of background fluorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond diffraction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-diffractive biological structures were imaged with a lateral resolution of â1/4150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.

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KW - Super Resolution Microscopy

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An LED-based super resolution GPU implemented structured illumination microscope

Abstract

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Bibliographical note

Keywords

Access to Document

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