An immunoelectrochemical platform for the biosensing of ‘Cocaine use’

Tulay Yilmaz Sengel; Emine Guler; Z. Pinar Gumus; Ebru Aldemir; Hakan Coskunol; Huseyin Akbulut; Demet Goen Colak; Ioan Cianga; Shuhei Yamada; Suna Timur; Takeshi Endo; Yusuf Yagci

doi:10.1016/j.snb.2017.02.087

An immunoelectrochemical platform for the biosensing of ‘Cocaine use’

Tulay Yilmaz Sengel, Emine Guler, Z. Pinar Gumus, Ebru Aldemir, Hakan Coskunol, Huseyin Akbulut, Demet Goen Colak, Ioan Cianga, Shuhei Yamada, Suna Timur^*, Takeshi Endo, Yusuf Yagci

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

In the present work, we demonstrate a novel surface design by combination of benzoylecgonine (BE) antibody and poly-L-phenylalanine bearing electroactive macromonomer (EDOT-BTDA-PPhe). This system served as an electro-immunosensor platform for the analysis of cocaine use by detecting both BE and cocaine. To construct the immunosensor, a glassy carbon electrode was covered with EDOT-BTDA-PPhe. Subsequently, BE antibody was immobilized on the polypeptide chains. The surface modifications were confirmed by electrochemical techniques. The immunosensor was used for the electrochemical detection of BE and cocaine as evidence of drug addiction. For both analytes, linearity was obtained between 0.5–25 μM. Finally, the proposed system was successfully employed for the analysis of synthetic biological fluids.

Original language	English
Pages (from-to)	310-318
Number of pages	9
Journal	Sensors and Actuators, B: Chemical
Volume	246
DOIs	https://doi.org/10.1016/j.snb.2017.02.087
Publication status	Published - 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

Abused drug detection
Biochemical screening tool
Cocaine
Immunosensor
Surface modification

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2017.02.087

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@article{e227e1c80cb245ed881fdb7401003f46,

title = "An immunoelectrochemical platform for the biosensing of {\textquoteleft}Cocaine use{\textquoteright}",

abstract = "In the present work, we demonstrate a novel surface design by combination of benzoylecgonine (BE) antibody and poly-L-phenylalanine bearing electroactive macromonomer (EDOT-BTDA-PPhe). This system served as an electro-immunosensor platform for the analysis of cocaine use by detecting both BE and cocaine. To construct the immunosensor, a glassy carbon electrode was covered with EDOT-BTDA-PPhe. Subsequently, BE antibody was immobilized on the polypeptide chains. The surface modifications were confirmed by electrochemical techniques. The immunosensor was used for the electrochemical detection of BE and cocaine as evidence of drug addiction. For both analytes, linearity was obtained between 0.5–25 μM. Finally, the proposed system was successfully employed for the analysis of synthetic biological fluids.",

keywords = "Abused drug detection, Biochemical screening tool, Cocaine, Immunosensor, Surface modification",

author = "{Yilmaz Sengel}, Tulay and Emine Guler and Gumus, {Z. Pinar} and Ebru Aldemir and Hakan Coskunol and Huseyin Akbulut and {Goen Colak}, Demet and Ioan Cianga and Shuhei Yamada and Suna Timur and Takeshi Endo and Yusuf Yagci",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2017",

doi = "10.1016/j.snb.2017.02.087",

language = "English",

volume = "246",

pages = "310--318",

journal = "Sensors and Actuators, B: Chemical",

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T1 - An immunoelectrochemical platform for the biosensing of ‘Cocaine use’

AU - Yilmaz Sengel, Tulay

AU - Guler, Emine

AU - Gumus, Z. Pinar

AU - Aldemir, Ebru

AU - Coskunol, Hakan

AU - Akbulut, Huseyin

AU - Goen Colak, Demet

AU - Cianga, Ioan

AU - Yamada, Shuhei

AU - Timur, Suna

AU - Endo, Takeshi

AU - Yagci, Yusuf

PY - 2017

Y1 - 2017

N2 - In the present work, we demonstrate a novel surface design by combination of benzoylecgonine (BE) antibody and poly-L-phenylalanine bearing electroactive macromonomer (EDOT-BTDA-PPhe). This system served as an electro-immunosensor platform for the analysis of cocaine use by detecting both BE and cocaine. To construct the immunosensor, a glassy carbon electrode was covered with EDOT-BTDA-PPhe. Subsequently, BE antibody was immobilized on the polypeptide chains. The surface modifications were confirmed by electrochemical techniques. The immunosensor was used for the electrochemical detection of BE and cocaine as evidence of drug addiction. For both analytes, linearity was obtained between 0.5–25 μM. Finally, the proposed system was successfully employed for the analysis of synthetic biological fluids.

AB - In the present work, we demonstrate a novel surface design by combination of benzoylecgonine (BE) antibody and poly-L-phenylalanine bearing electroactive macromonomer (EDOT-BTDA-PPhe). This system served as an electro-immunosensor platform for the analysis of cocaine use by detecting both BE and cocaine. To construct the immunosensor, a glassy carbon electrode was covered with EDOT-BTDA-PPhe. Subsequently, BE antibody was immobilized on the polypeptide chains. The surface modifications were confirmed by electrochemical techniques. The immunosensor was used for the electrochemical detection of BE and cocaine as evidence of drug addiction. For both analytes, linearity was obtained between 0.5–25 μM. Finally, the proposed system was successfully employed for the analysis of synthetic biological fluids.

KW - Abused drug detection

KW - Biochemical screening tool

KW - Cocaine

KW - Immunosensor

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=85013642362&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2017.02.087

DO - 10.1016/j.snb.2017.02.087

M3 - Article

AN - SCOPUS:85013642362

SN - 0925-4005

VL - 246

SP - 310

EP - 318

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

An immunoelectrochemical platform for the biosensing of ‘Cocaine use’

Abstract

Bibliographical note

Keywords

UN SDGs

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