Abstract
Polyethylene glycol (PEG) hydrogels that have natural fibers mimicking extracellular matrix can be used as a model to understand the role of substrate properties on cell growth and migration. Due to the dependence of cell movement to adhesion, characterization of motility is needed to prepare biocompatible substrates. We demonstrated a method to encapsulate collagen into PEG hydrogel crosslinked via photopolymerization and studied the effect of fiber density on motility dynamics. Porous hydrogel immersed into collagen solution was coated with fibers after neutralizing solution. We provided a detailed study of cell instantaneous/average speed, total displacement, persistence and angular displacement. We found that cells demonstrated a biphasic motility where a maximum speed of 17.4 μm/h with a total distance of 215 μm and persistence of 0.43 were obtained at 1.2 mg/ml collagen. High occurrence of low angular displacement observed at intermediate fiber density suggests that cells tend to move forward along hydrogels. Increased anisotropy at low density was an indication of forward and backward movement. Finally, matrix deformation was determined in the absence of fluorescent beads by tracking fiber displacement at subpixel resolution. Our findings establish a method for preparation of collagen coated hydrogels and provide an insight into cell motility dynamics.
| Original language | English |
|---|---|
| Pages (from-to) | 414-423 |
| Number of pages | 10 |
| Journal | International Journal of Biological Macromolecules |
| Volume | 157 |
| DOIs | |
| Publication status | Published - 15 Aug 2020 |
Bibliographical note
Publisher Copyright:© 2020
Funding
This work was partially supported by grant 112T823 from TUBITAK . O.B.A. was supported by 2210 national scholarship predoctoral training program. We thank Prof. B. Erdem Alaca and Naveed Salman at Koç University for the use of uniaxial stretcher and tensile measurements. We are very grateful to Olaf Pharmaceuticals for providing purified type I collagen and Zeynep Kaya for preparing GFP expressing 293T cells. We thank the Surface Science Research Center at Koç University for assistance to use of scanning electron microscope. This work was partially supported by grant 112T823 from TUBITAK. O.B.A. was supported by 2210 national scholarship predoctoral training program. We thank Prof. B. Erdem Alaca and Naveed Salman at Ko? University for the use of uniaxial stretcher and tensile measurements. We are very grateful to Olaf Pharmaceuticals for providing purified type I collagen and Zeynep Kaya for preparing GFP expressing 293T cells. We thank the Surface Science Research Center at Ko? University for assistance to use of scanning electron microscope.
| Funders | Funder number |
|---|---|
| 2210 national scholarship predoctoral training program | |
| Surface Science Research Center | |
| TUBITAK |
Keywords
- Collagen
- Hydrogel
- Motility dynamics
- PEG
- Persistence
- Single-cell tracking