Accurate determination of amino acids by quadruple isotope dilution-reverse phase liquid Chromatography-Tandem mass spectrometry after derivatization with 2-Naphthoyl chloride

Elif Öztürk Er*, Sezin Erarpat, Süleyman Bodur, Ömer Tahir Günkara, Belma Özbek, Sezgin Bakırdere

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The determination of amino acids in biological samples is central to the diagnosis of inherited metabolic disorders and also gives significant information about the metabolisms in the cells and living body. The development of analytical method for reliable quantification of amino acids in biological samples is still challenging because of the polar nature of amino acids and complex nature of biological samples causing a high degree of interferences during analysis. In the present study, a pre-column derivatization method using 2-naphtoyl chloride combined with liquid chromatography-tandem mass spectrometry method was developed for the determination of 17 amino acids in human serum and urine matrices. Low detection limits were obtained in the range of 0.015 – 0.266 µmol kg−1 and acceptable recovery results were obtained in human serum and urine samples. Isotopically labelled (15N labelled) amino acids were spiked to standards and samples before derivatization to compensate for the analytical errors in the whole procedure. The combination of quadrupole isotope dilution strategy with the derivatization based reversed phase chromatography allowed to improve method accuracy and precision.

Original languageEnglish
Article number462870
JournalJournal of Chromatography A
Volume1667
DOIs
Publication statusPublished - 29 Mar 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022 Elsevier B.V.

Funding

This study was supported by Yildiz Technical University. Project Number: FDK-2017–3186.

FundersFunder number
Yildiz Teknik ÜniversitesiFDK-2017–3186

    Keywords

    • Amino acids
    • Derivatization
    • Human serum
    • Human urine
    • Isotope dilution

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