A novel exopolysaccharide from cold-adapted yeast Rhodotorula glutinis, along with structural, rheological, antioxidant, and antibiofilm properties

Sebahat Oztekin, Dilara Nur Dikmetas, Funda Karbancıoglu-Guler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In the present study, a novel exopolysaccharide EPS-BMD26 produced by cold-adapted yeast Rhodotorula glutinis was structurally examined, along with its rheological, antioxidant, and antibiofilm properties. High-performance liquid chromatography (HPLC) analysis revealed glucose and galactose as two monosaccharide constituents. The functional groups within the EPS-BMD26 structure were detected by Fourier transform infrared (FT-IR) spectroscopy. The 1H and 13C nuclear magnetic resonance (NMR) spectra of EPS-BMD26 also confirmed its β-D-glucan type with galactose residues with an average molecular weight of 118 kDa. Differential scanning calorimetry (DSC) analysis revealed that EPS-BMD26 had a high level of thermal stability, up to 326.16 °C. Scanning electron microscopy (SEM) analysis demonstrated that EPS-BMD26 had a porous microstructure with fissures. X-ray diffraction (XRD) analysis revealed its semi-crystalline nature. EPS-BMD26 showed moderate and concentration-dependent antioxidant potential recruiting different methods, including •OH, DPPH•, CUPRAC, and ABTS radical scavenging activities. Water-soluble EPS-BMD26 exhibited high water holding capacity of 190 ± 0.22% with a water solubility index of 60.6 ± 8.3%. At 1.25 mg mL-1, EPS-BMD26 inhibited biofilm formation of Staphylococcus aureus ATCC 25923 by 79.5%. Rheological characterization revealed that the aqueous solution of EPS-BMD26 had shear thinning and pseudoplastic behavior. Given these properties, EPS-BMD26 may be a promising bio-hydrocolloid, antibiofilm agent, and food additive for industrial applications.

Original languageEnglish
Pages (from-to)1507-1523
Number of pages17
JournalBiomass Conversion and Biorefinery
Volume15
Issue number1
DOIs
Publication statusPublished - Jan 2025

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.

Funding

This work was funded by the Scientific Research Council of Istanbul Technical University (Grant No. 40878).

FundersFunder number
Istanbul Teknik Üniversitesi40878

    Keywords

    • Antibiofilm
    • Antioxidant
    • Exopolysaccharide
    • Rheology
    • Rhodotorula glutinis
    • Structural analysis

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