Abstract
A neutral ginger polysaccharide fraction (NGP) was isolated from ginger, and its primary structures and immunomodulatory activity were investigated. The results showed that NGP had a low molecular weight of 6305 Da. NGP principally consisted of glucose (93.88%) together with minor levels of galactose (3.27%) and arabinose (1.67%). Besides, results of methylation analysis and 1D/2D NMR spectroscopy demonstrated that NGP was α-glucan which had the main chain of 1,4-linked α-D-Glcp and α-D-Glcp residues branched at C-6 position which was different from the common triple helical β-glucans. NGP also displayed a remarkable immunological activity on the RAW264.7 cells in vitro. It could significantly enhance the proliferation of macrophages without cytotoxicity and increase the production of immune substances (NO, TNF-α, IL-1β and IL-6). The secretion at the concentration of 200 μg/mL was 29.41 μM, 1496.71, 44.30 and 1889.83 pg/mL for each substance, respectively. The results indicated that NGP could be a potential immune agent and might provide meaningful information for further chain conformation and immune mechanism research.
Original language | English |
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Article number | 129261 |
Journal | Food Chemistry |
Volume | 350 |
DOIs | |
Publication status | Published - 15 Jul 2021 |
Bibliographical note
Publisher Copyright:© 2021 Elsevier Ltd
Funding
The project was supported by Major Agricultural Applied Technology Innovation Project of Shandong Province in 2018 (39797), Major Scientific and Technological Innovation Projects of Key R&D Program of Shandong Province (2019JZZY020607).
Funders | Funder number |
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Major Agricultural Applied Technology Innovation Project of Shandong Province | 39797 |
Major Scientific and Technological Innovation Projects of Key R&D Program of Shandong Province | 2019JZZY020607 |
Keywords
- Ginger polysaccharide
- Immunomodulatory activity
- Structure characterization
- Triple helix structure
- α-(1→4)-glucan