A neutral polysaccharide with a triple helix structure from ginger: Characterization and immunomodulatory activity

Xiaolong Yang, Siqing Wei, Xiaoming Lu, Xuguang Qiao, Jesus Simal-Gandara, Esra Capanoglu, Łukasz Woźniak, Liang Zou, Hui Cao, Jianbo Xiao*, Xiaozhen Tang, Ningyang Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

89 Citations (Scopus)

Abstract

A neutral ginger polysaccharide fraction (NGP) was isolated from ginger, and its primary structures and immunomodulatory activity were investigated. The results showed that NGP had a low molecular weight of 6305 Da. NGP principally consisted of glucose (93.88%) together with minor levels of galactose (3.27%) and arabinose (1.67%). Besides, results of methylation analysis and 1D/2D NMR spectroscopy demonstrated that NGP was α-glucan which had the main chain of 1,4-linked α-D-Glcp and α-D-Glcp residues branched at C-6 position which was different from the common triple helical β-glucans. NGP also displayed a remarkable immunological activity on the RAW264.7 cells in vitro. It could significantly enhance the proliferation of macrophages without cytotoxicity and increase the production of immune substances (NO, TNF-α, IL-1β and IL-6). The secretion at the concentration of 200 μg/mL was 29.41 μM, 1496.71, 44.30 and 1889.83 pg/mL for each substance, respectively. The results indicated that NGP could be a potential immune agent and might provide meaningful information for further chain conformation and immune mechanism research.

Original languageEnglish
Article number129261
JournalFood Chemistry
Volume350
DOIs
Publication statusPublished - 15 Jul 2021

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Ltd

Funding

The project was supported by Major Agricultural Applied Technology Innovation Project of Shandong Province in 2018 (39797), Major Scientific and Technological Innovation Projects of Key R&D Program of Shandong Province (2019JZZY020607).

FundersFunder number
Major Agricultural Applied Technology Innovation Project of Shandong Province39797
Major Scientific and Technological Innovation Projects of Key R&D Program of Shandong Province2019JZZY020607

    Keywords

    • Ginger polysaccharide
    • Immunomodulatory activity
    • Structure characterization
    • Triple helix structure
    • α-(1→4)-glucan

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