Abstract
Poly(styrene-divinylbenzene) (PS-DVB) microspheres were grafted with polystyrene (PS) via surface initiated-atom transfer radical polymerization (SI-ATRP). The grafted PS chains were sulfonated with H 2SO 4 in the presence of P 2O 5. The sulfonic acid groups (4.8mmol/g) on the surface brushes were neutralized with aniline, and the adsorbed aniline was polymerized by oxidizing with potassium persulfate to give self-doped and thick PANI layers (i.e. 16μm) on the microspheres. Then, cellulase was immobilized on the polyaniline (PANI) coated PS-DVB-g-PS microspheres via adsorption and adsorption/cross-linking methods. The properties of the immobilized cellulase preparations were investigated and compared with those of the free enzyme. After immobilization, the recovered activities of the adsorbed and adsorbed/cross-linked cellulase were found to be 73 and 62% for the substrate, carboxymethyl cellulose (CMC, 1.0g/L), respectively. The immobilized enzyme preparations had better stabilities and higher retained activities with respect to pH, temperature and storage stability than those of the free enzyme.
Original language | English |
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Pages (from-to) | 404-412 |
Number of pages | 9 |
Journal | Chemical Engineering Journal |
Volume | 189-190 |
DOIs | |
Publication status | Published - 1 May 2012 |
Keywords
- Cellulase
- Cellulose
- Microspheres
- Oxidative polymerization
- Polyaniline
- SI-ATRP