A methionine biomolecule-modified chromenylium-cyanine fluorescent probe for the analysis of Hg2+ in the environment and living cells

Yusuf Alcay, Emre Ozdemir, Mustafa Semih Yildirim, Utku Ertugral, Ozgur Yavuz, Hulya Aribuga, Yilmaz Ozkilic, Nurcan Şenyurt Tuzun, Ayse Buse Ozdabak Sert, Fatma Nese Kok, Ismail Yilmaz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The objective of the study is, for the first time, to construct a new near infrared (NIR) fluorophore, spectrophotometric, colorimetric, ratiometric, and turn-on probe (CSME) based on chromenylium cyanine platform decorated with methionine biomolecule to provide an efficient solution for critical shortcoming to be encountered for analysis of hazardous Hg2+ in environment and living cell. The CSME structure and its interaction with Hg2+ ion were evaluated by NMR, FTIR, MS, UV–Vis and fluorescence methods as well as Density Functional Theory (DFT) calculations. The none fluorescence CSME having spirolactam ring only interacted with Hg2+ in aqueous solution including competing ions. This interaction caused the fluorescence CSME with opened spirolactam form which exhibited spectral and colorimetric changes in the NIR region. The probe based on UV–Vis and fluorescence techniques respond in 90 s, has wide linear ranges (for UV–Vis: 6.29 × 10−8 – 1.86 × 10−4 M; for fluorescence: 9.49 × 10−9 – 1.13 × 10−5 M), and has a lower Limit of Detection (LOD) value (for fluorescence: 4.93 × 10−9 M, 0.99 ng/mL) than the value predicted by the US Environmental Protection Agency (EPA) organization. Hg2+ analysis was performed in drinking and tap water with low Relative Standard Deviation (RSD) values and high recovery. Smartphone and living cell applications were successfully performed for colorimetric sensing Hg2+ in real samples and 3T3 cells, respectively.

Original languageEnglish
Article number124471
JournalTalanta
Volume259
DOIs
Publication statusPublished - 1 Jul 2023

Bibliographical note

Publisher Copyright:
© 2023 Elsevier B.V.

Funding

This work was supported by The Scientific and Technical Research Council of Turkey ( TÜBİTAK , Project no: 219Z085 ). Computing resources used in this work were provided by the National Center for High Performance Computing of Turkey (UHeM) under grant number 1011382021.

FundersFunder number
National Center for High Performance Computing of Turkey
Ulusal Yüksek Başarımlı Hesaplama Merkezi, Istanbul Teknik Üniversitesi1011382021
Türkiye Bilimsel ve Teknolojik Araştırma Kurumu219Z085

    Keywords

    • Chromenylium cyanine
    • Density functional theory
    • Hazardous
    • Mercury
    • Near-IR probe
    • Ratiometric sensor

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