3D macromolecular structure analyses: Applications in plant proteins

Filiz Dede; Gizem Dinler; Zehra Sayers

doi:10.1007/978-1-4020-5724-3_12

3D macromolecular structure analyses: Applications in plant proteins

Filiz Dede^*, Gizem Dinler, Zehra Sayers

^*Corresponding author for this work

Sabanci University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

Attempts to relate the function to the structural features of biological macromolecules have intensified through the use of synchrotron radiation (SR) based techniques. Small Angle X-ray Scattering (SXAS) is a technique that has become readily accessible through several bamlines on SR sources all over the world. SAXS is used for obtaining low resolution structural information and is particularly useful for macromolecules that do not easily crystallize or alternatively when time-resolved structural information is required. In this paper use of SAXS for determination of structural parameters of a wheat metallothionein as a fusion protein with glutathione-stransferase (GST) is presented together with low resolution models. Results are discussed in the framework of functional roles of wheat metallothionein.

Original language	English
Title of host publication	Brilliant Light in Life and Material Sciences
Publisher	Springer Verlag
Pages	141-151
Number of pages	11
ISBN (Print)	9781402057229
DOIs	https://doi.org/10.1007/978-1-4020-5724-3_12
Publication status	Published - 2007
Externally published	Yes

Publication series

Name	NATO Security through Science Series B: Physics and Biophysics
ISSN (Print)	1871-465X

Keywords

Metallothionein
SAXS
Synchrotron radiation

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10.1007/978-1-4020-5724-3_12

Cite this

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title = "3D macromolecular structure analyses: Applications in plant proteins",

abstract = "Attempts to relate the function to the structural features of biological macromolecules have intensified through the use of synchrotron radiation (SR) based techniques. Small Angle X-ray Scattering (SXAS) is a technique that has become readily accessible through several bamlines on SR sources all over the world. SAXS is used for obtaining low resolution structural information and is particularly useful for macromolecules that do not easily crystallize or alternatively when time-resolved structural information is required. In this paper use of SAXS for determination of structural parameters of a wheat metallothionein as a fusion protein with glutathione-stransferase (GST) is presented together with low resolution models. Results are discussed in the framework of functional roles of wheat metallothionein.",

keywords = "Metallothionein, SAXS, Synchrotron radiation",

author = "Filiz Dede and Gizem Dinler and Zehra Sayers",

year = "2007",

doi = "10.1007/978-1-4020-5724-3_12",

language = "English",

isbn = "9781402057229",

series = "NATO Security through Science Series B: Physics and Biophysics",

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T2 - Applications in plant proteins

AU - Dede, Filiz

AU - Dinler, Gizem

AU - Sayers, Zehra

PY - 2007

Y1 - 2007

N2 - Attempts to relate the function to the structural features of biological macromolecules have intensified through the use of synchrotron radiation (SR) based techniques. Small Angle X-ray Scattering (SXAS) is a technique that has become readily accessible through several bamlines on SR sources all over the world. SAXS is used for obtaining low resolution structural information and is particularly useful for macromolecules that do not easily crystallize or alternatively when time-resolved structural information is required. In this paper use of SAXS for determination of structural parameters of a wheat metallothionein as a fusion protein with glutathione-stransferase (GST) is presented together with low resolution models. Results are discussed in the framework of functional roles of wheat metallothionein.

AB - Attempts to relate the function to the structural features of biological macromolecules have intensified through the use of synchrotron radiation (SR) based techniques. Small Angle X-ray Scattering (SXAS) is a technique that has become readily accessible through several bamlines on SR sources all over the world. SAXS is used for obtaining low resolution structural information and is particularly useful for macromolecules that do not easily crystallize or alternatively when time-resolved structural information is required. In this paper use of SAXS for determination of structural parameters of a wheat metallothionein as a fusion protein with glutathione-stransferase (GST) is presented together with low resolution models. Results are discussed in the framework of functional roles of wheat metallothionein.

KW - Metallothionein

KW - SAXS

KW - Synchrotron radiation

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U2 - 10.1007/978-1-4020-5724-3_12

DO - 10.1007/978-1-4020-5724-3_12

M3 - Conference contribution

AN - SCOPUS:85099734584

SN - 9781402057229

T3 - NATO Security through Science Series B: Physics and Biophysics

SP - 141

EP - 151

BT - Brilliant Light in Life and Material Sciences

PB - Springer Verlag

ER -

3D macromolecular structure analyses: Applications in plant proteins

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